| Exploring the photosynthetic production capacity of sucrose by cyanobacteria | |
Du, Wei1,2; Liang, Feiyan1,2 ; Duan, Yangkai1,2; Tan, Xiaoming1; Lu, Xuefeng1
| |
| 2013-09-01 | |
| 发表期刊 | METABOLIC ENGINEERING
 |
| 卷号 | 19期号:2013页码:17-25 |
| 摘要 | Because cyanobacteria are photosynthetic, fast-growing microorganisms that can accumulate sucrose under salt stress, they have a potential application as a sugar source for the biomass-derived production of renewable fuels and chemicals. In the present study, the production of sucrose by the cyanobacteria Synechocystis sp. PCC6803, Synechococcus elongatus PCC7942, and Anabaena sp. PCC7120 was examined. The three species displayed different growth curves and intracellular sucrose accumulation rates in response to NaCl. Synechocystis sp. PCC6803 was used to examine the impact of modifying the metabolic pathway on the levels of sucrose production. The co-overexpression of sps (slr0045), spp (slr0953), and ugp (slr0207) lead to a 2-fold increase in intracellular sucrose accumulation, whereas knockout of ggpS (sll1566) resulted in a 1.5-fold increase in the production of this sugar When combined, these genetic modifications resulted in a fourfold increase in intracellular sucrose accumulation. To explore methods for optimizing the transport of the intracellular sucrose to the growth medium, the acid-wash technique and the CscB (sucrose permease)-dependent export method were evaluated using Synechocystis sp. PCC6803. Whereas the acid-wash technique proved to be effective, the CscB-dependent export method was not effective. Taken together, these results suggest that using genetic engineering, photosynthetic cyanobacteria can be optimized for efficient sucrose production. (C) 2013 Elsevier Inc. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Sucrose Glucosylglycerol Ion-exchange Chromatography Synechocystis Sp Pcc6803 Synechococcus Elongatus Pcc7942 Anabaena Sp Pcc7120 |
| 学科领域 | 生物代谢工程 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.ymben.2013.05.001 |
| 关键词[WOS] | SYNECHOCYSTIS-SP PCC-6803 ; ESCHERICHIA-COLI ; COMPATIBLE SOLUTE ; SALT ACCLIMATION ; ACCUMULATION ; GLUCOSYLGLYCEROL ; OSMOLYTE ; STRESS ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000324162100003 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1622 |
| 专题 | 微生物代谢工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Wei,Liang, Feiyan,Duan, Yangkai,et al. Exploring the photosynthetic production capacity of sucrose by cyanobacteria[J]. METABOLIC ENGINEERING,2013,19(2013):17-25. |
| APA | Du, Wei,Liang, Feiyan,Duan, Yangkai,Tan, Xiaoming,&Lu, Xuefeng.(2013).Exploring the photosynthetic production capacity of sucrose by cyanobacteria.METABOLIC ENGINEERING,19(2013),17-25. |
| MLA | Du, Wei,et al."Exploring the photosynthetic production capacity of sucrose by cyanobacteria".METABOLIC ENGINEERING 19.2013(2013):17-25. |
| 条目包含的文件 | 下载所有文件 | |||||
| 文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
| Exploring the photos（2383KB） | 开放获取 | CC BY-NC-SA | 浏览 下载 | |||
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论