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Structure and regulation of the cellulose degradome in Clostridium cellulolyticum
Xu, Chenggang1,2; Huang, Ranran1,2; Teng, Lin1,2; Wang, Dongmei1,2; Hemme, Christopher L.3; Borovok, Ilya4; He, Qiang5; Lamed, Raphael4; Bayer, Edward A.6; Zhou, Jizhong3; Xu, Jian1,2
2013-05-08
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
Volume6Issue:1Pages:1-15
Abstract Background: Many bacteria efficiently degrade lignocellulose yet the underpinning genome-wide metabolic and regulatory networks remain elusive. Here we revealed the “cellulose degradome” for the model mesophilic cellulolytic bacterium Clostridium cellulolyticum ATCC 35319, via an integrated analysis of its complete genome, its transcriptomes under glucose, xylose, cellobiose, cellulose, xylan or corn stover and its extracellular proteomes under glucose, cellobiose or cellulose.
Results: Proteins for core metabolic functions, environment sensing, gene regulation and polysaccharide metabolism were enriched in the cellulose degradome. Analysis of differentially expressed genes revealed a “core”set of 48 CAZymes required for degrading cellulose-containing substrates as well as an “accessory” set of 76 CAZymes required for specific non-cellulose substrates. Gene co-expression analysis suggested that Carbon
Catabolite Repression (CCR) related regulators sense intracellular glycolytic intermediates and control the core CAZymes that mainly include cellulosomal components, whereas 11 sets of Two-Component Systems (TCSs) respond to availability of extracellular soluble sugars and respectively regulate most of the accessory CAZymes and associated transporters. Surprisingly, under glucose alone, the core cellulases were highly expressed at both
transcript and protein levels. Furthermore, glucose enhanced cellulolysis in a dose-dependent manner, via inducing cellulase transcription at low concentrations.
Conclusion: A molecular model of cellulose degradome in C. cellulolyticum (Ccel) was proposed, which revealed the substrate-specificity of CAZymes and the transcriptional regulation of core cellulases by CCR where the glucose acts as a CCR inhibitor instead of a trigger. These features represent a distinct environment-sensing strategy for competing while collaborating for cellulose utilization, which can be exploited for process and genetic engineering of microbial cellulolysis.
; Background: Many bacteria efficiently degrade lignocellulose yet the underpinning genome-wide metabolic and regulatory networks remain elusive. Here we revealed the "cellulose degradome" for the model mesophilic cellulolytic bacterium Clostridium cellulolyticum ATCC 35319, via an integrated analysis of its complete genome, its transcriptomes under glucose, xylose, cellobiose, cellulose, xylan or corn stover and its extracellular proteomes under glucose, cellobiose or cellulose.
SubtypeArticle
KeywordCellulose Degradation Transcription Two-component Systems Catabolite Control Proteins Ccpa-like Lacl Family
Subject Area功能基因组
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1186/1754-6834-6-73
WOS KeywordCATABOLITE-RESPONSIVE ELEMENTS ; BACILLUS-SUBTILIS ; TRANSCRIPTIONAL ANALYSIS ; HEMICELLULASE GENES ; GENOME SEQUENCE ; THERMOCELLUM ; REPRESSION ; BACTERIA ; EXPRESSION ; ETHANOL
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000319357600001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/1620
Collection单细胞中心组群
Affiliation1.Chinese Acad Sci, CAS Key Lab Biofuels, BioEnergy Genome Ctr, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
3.Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73072 USA
4.Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, IL-69978 Ramat Aviv, Israel
5.Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA
6.Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel
Recommended Citation
GB/T 7714
Xu, Chenggang,Huang, Ranran,Teng, Lin,et al. Structure and regulation of the cellulose degradome in Clostridium cellulolyticum[J]. BIOTECHNOLOGY FOR BIOFUELS,2013,6(1):1-15.
APA Xu, Chenggang.,Huang, Ranran.,Teng, Lin.,Wang, Dongmei.,Hemme, Christopher L..,...&Xu, Jian.(2013).Structure and regulation of the cellulose degradome in Clostridium cellulolyticum.BIOTECHNOLOGY FOR BIOFUELS,6(1),1-15.
MLA Xu, Chenggang,et al."Structure and regulation of the cellulose degradome in Clostridium cellulolyticum".BIOTECHNOLOGY FOR BIOFUELS 6.1(2013):1-15.
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