| Structure and regulation of the cellulose degradome in Clostridium cellulolyticum | |
Xu, Chenggang1,2 ; Huang, Ranran1,2; Teng, Lin1,2; Wang, Dongmei1,2; Hemme, Christopher L.3; Borovok, Ilya4; He, Qiang5; Lamed, Raphael4; Bayer, Edward A.6; Zhou, Jizhong3; Xu, Jian1,2
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| 2013-05-08 | |
| 发表期刊 | BIOTECHNOLOGY FOR BIOFUELS
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| 卷号 | 6期号:1页码:1-15 |
| 摘要 | Background: Many bacteria efficiently degrade lignocellulose yet the underpinning genome-wide metabolic and regulatory networks remain elusive. Here we revealed the “cellulose degradome” for the model mesophilic cellulolytic bacterium Clostridium cellulolyticum ATCC 35319, via an integrated analysis of its complete genome, its transcriptomes under glucose, xylose, cellobiose, cellulose, xylan or corn stover and its extracellular proteomes under glucose, cellobiose or cellulose. Results: Proteins for core metabolic functions, environment sensing, gene regulation and polysaccharide metabolism were enriched in the cellulose degradome. Analysis of differentially expressed genes revealed a “core”set of 48 CAZymes required for degrading cellulose-containing substrates as well as an “accessory” set of 76 CAZymes required for specific non-cellulose substrates. Gene co-expression analysis suggested that Carbon Catabolite Repression (CCR) related regulators sense intracellular glycolytic intermediates and control the core CAZymes that mainly include cellulosomal components, whereas 11 sets of Two-Component Systems (TCSs) respond to availability of extracellular soluble sugars and respectively regulate most of the accessory CAZymes and associated transporters. Surprisingly, under glucose alone, the core cellulases were highly expressed at both transcript and protein levels. Furthermore, glucose enhanced cellulolysis in a dose-dependent manner, via inducing cellulase transcription at low concentrations. Conclusion: A molecular model of cellulose degradome in C. cellulolyticum (Ccel) was proposed, which revealed the substrate-specificity of CAZymes and the transcriptional regulation of core cellulases by CCR where the glucose acts as a CCR inhibitor instead of a trigger. These features represent a distinct environment-sensing strategy for competing while collaborating for cellulose utilization, which can be exploited for process and genetic engineering of microbial cellulolysis. ; Background: Many bacteria efficiently degrade lignocellulose yet the underpinning genome-wide metabolic and regulatory networks remain elusive. Here we revealed the "cellulose degradome" for the model mesophilic cellulolytic bacterium Clostridium cellulolyticum ATCC 35319, via an integrated analysis of its complete genome, its transcriptomes under glucose, xylose, cellobiose, cellulose, xylan or corn stover and its extracellular proteomes under glucose, cellobiose or cellulose. |
| 文章类型 | Article |
| 关键词 | Cellulose Degradation Transcription Two-component Systems Catabolite Control Proteins Ccpa-like Lacl Family |
| 学科领域 | 功能基因组 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
| DOI | 10.1186/1754-6834-6-73 |
| 关键词[WOS] | CATABOLITE-RESPONSIVE ELEMENTS ; BACILLUS-SUBTILIS ; TRANSCRIPTIONAL ANALYSIS ; HEMICELLULASE GENES ; GENOME SEQUENCE ; THERMOCELLUM ; REPRESSION ; BACTERIA ; EXPRESSION ; ETHANOL |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS类目 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| WOS记录号 | WOS:000319357600001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1620 |
| 专题 | 单细胞中心组群 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Biofuels, BioEnergy Genome Ctr, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 3.Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73072 USA 4.Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, IL-69978 Ramat Aviv, Israel 5.Univ Tennessee, Dept Civil & Environm Engn, Knoxville, TN 37996 USA 6.Weizmann Inst Sci, Dept Biol Chem, IL-76100 Rehovot, Israel |
| 推荐引用方式 GB/T 7714 | Xu, Chenggang,Huang, Ranran,Teng, Lin,et al. Structure and regulation of the cellulose degradome in Clostridium cellulolyticum[J]. BIOTECHNOLOGY FOR BIOFUELS,2013,6(1):1-15. |
| APA | Xu, Chenggang.,Huang, Ranran.,Teng, Lin.,Wang, Dongmei.,Hemme, Christopher L..,...&Xu, Jian.(2013).Structure and regulation of the cellulose degradome in Clostridium cellulolyticum.BIOTECHNOLOGY FOR BIOFUELS,6(1),1-15. |
| MLA | Xu, Chenggang,et al."Structure and regulation of the cellulose degradome in Clostridium cellulolyticum".BIOTECHNOLOGY FOR BIOFUELS 6.1(2013):1-15. |
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