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Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter
Lin, Lu1,2; Ji, Yuetong1,2; Tu, Qichao3,4; Huang, Ranran1,2; Teng, Lin1,2; Zeng, Xiaowei1,2; Song, Houhui1,2; Wang, Kun1,2; Zhou, Qian1,2; Li, Yifei1,2; Cui, Qiu1,2; He, Zhili3,4; Zhou, Jizhong3,4; Xu, Jian1,2
2013-07-22
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
Volume6Issue:1Pages:1-17
Abstract Introduction: The molecular links between shock-response and adaptation remain poorly understood, particularly for extremophiles. This has hindered rational engineering of solvent tolerance and correlated traits (e.g., productivity) in extremophiles. To untangle such molecular links, here we established a model that tracked the microevolution from shock to adaptation in thermophilic bacteria.
Method: Temporal dynamics of genomes and transcriptomes was tracked for Thermoanaerobacter sp. X514 which under increasing exogenous ethanol evolved from ethanol-sensitive wild-type (Strain X) to tolerance of 2%- (XI) and eventually 6%-ethanol (XII). Based on the reconstructed transcriptional network underlying stress tolerance, genetic engineering was employed to improve ethanol tolerance and production in Thermoanaerobacter.
Results: The spontaneous genome mutation rate (μg) of Thermoanaerobacter sp. X514, calculated at 0.045, suggested a higher mutation rate in thermophile than previously thought. Transcriptomic comparison revealed that shock-response and adaptation were distinct in nature, whereas the transcriptomes of XII resembled those of the extendedly shocked X. To respond to ethanol shock, X employed fructose-specific phosphotransferase system (PTS),
Arginine Deiminase (ADI) pathway, alcohol dehydrogenase (Adh) and a distinct mechanism of V-type ATPase. As an adaptation to exogenous ethanol, XI mobilized resistance-nodulation-cell division (RND) efflux system and Adh, whereas XII, which produced higher ethanol than XI, employed ECF-type ϭ24, an alcohol catabolism operon and phase-specific heat-shock proteins (Hsps), modulated hexose/pentose-transport operon structure and reinforced membrane rigidity. Exploiting these findings, we further showed that ethanol productivity and tolerance can be improved simultaneously by overexpressing adh or ϭ24 in X.
Conclusion: Our work revealed thermophilic-bacteria specific features of adaptive evolution and demonstrated a rational strategy to engineer co-evolving industrial traits. As improvements of shock-response, stress tolerance and productivity have been crucial aims in industrial applications employing thermophiles, our findings should be valuable not just to the production of ethanol but also to a wide variety of biofuels and biochemicals.
 
 
; Introduction: The molecular links between shock-response and adaptation remain poorly understood, particularly for extremophiles. This has hindered rational engineering of solvent tolerance and correlated traits (e. g., productivity) in extremophiles. To untangle such molecular links, here we established a model that tracked the microevolution from shock to adaptation in thermophilic bacteria.
SubtypeArticle
KeywordShock Adaptation Ethanol Microevolution Thermophile
Subject Area功能基因组
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine ; Technology
DOI10.1186/1754-6834-6-103
WOS KeywordESCHERICHIA-COLI ; CLOSTRIDIUM-ACETOBUTYLICUM ; BUTANOL STRESS ; SACCHAROMYCES-CEREVISIAE ; TRANSCRIPTIONAL ANALYSIS ; ISOBUTANOL TOLERANCE ; ADAPTIVE EVOLUTION ; SOLVENT TOLERANCE ; GENOMIC ANALYSIS ; SIGMA-FACTOR
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000323377300001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/1616
Collection单细胞中心组群
Affiliation1.Chinese Acad Sci, BioEnergy Genome Ctr, CAS Key Lab Biofuels, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao, Shandong, Peoples R China
3.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA
4.Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA
Recommended Citation
GB/T 7714
Lin, Lu,Ji, Yuetong,Tu, Qichao,et al. Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter[J]. BIOTECHNOLOGY FOR BIOFUELS,2013,6(1):1-17.
APA Lin, Lu.,Ji, Yuetong.,Tu, Qichao.,Huang, Ranran.,Teng, Lin.,...&Xu, Jian.(2013).Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter.BIOTECHNOLOGY FOR BIOFUELS,6(1),1-17.
MLA Lin, Lu,et al."Microevolution from shock to adaptation revealed strategies improving ethanol tolerance and production in Thermoanaerobacter".BIOTECHNOLOGY FOR BIOFUELS 6.1(2013):1-17.
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