|. Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production
|Lin, L.; Xu, J.
| Interest in thermophilic bacteria as live-cell catalysts in biofuel and biochemical industry has surged in recent years, due to their tolerance of high temperature and wide spectrum of carbon-sources that include cellulose. However their direct employment as microbial cellular factories in the highly demanding industrial conditions has been hindered by uncompetitive biofuel productivity, relatively low tolerance to solvent and osmic stresses, and limitation in genome engineering tools. In this work we reviewrecent advances in dissecting and engineering the metabolic and regulatory networks of thermophilic bacteria for improving the traits of key interest in biofuel industry: cellulose degradation, pentose–hexose co-utilization, and tolerance of thermal, osmotic, and solvent stresses.Moreover, newtechnologies enablingmore efficient genetic engineering of thermophileswere discussed, such as improved electroporation, ultrasound-mediated DNAdelivery, aswellas thermo-stableplasmids andfunctional selection systems. Expanded applications of such technological advancements in thermophilic microbes promise to substantiate a synthetic biology perspective,where functional parts,module, chassis, cells and consortia weremodularly designed and rationally assembled for themanymissions at industry and nature that demand the extraordinary talents of these extremophiles
Lin, L.,Xu, J.. . Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production[J]. Biotechnology Advance,2013,31(6):827-837.
Lin, L.,&Xu, J..(2013).. Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production.Biotechnology Advance,31(6),827-837.
Lin, L.,et al.". Dissecting and engineering metabolic and regulatory networks of thermophilic bacteria for biofuel production".Biotechnology Advance 31.6(2013):827-837.
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