| MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering | |
| Yu, Yanchong; Hu, Ruibo; Wang, Huamei; Cao, Yingping; He, Guo; Fu, Chunxiang; Zhou, Gongke | |
| 2013-11-01 | |
| 发表期刊 | PLANT SCIENCE
 |
| 卷号 | 212期号:212页码:1-9 |
| 摘要 | WRKY proteins play crucial roles in various plant processes. An AtWRKY12 homologous gene, namedMlWRKY12, was isolated from Miscanthus lutarioriparius. The MlWRKY12 gene encodes a WRKY transcrip-tion factor belonging to the group IIc subfamily. MlWRKY12 is a nuclear protein. Gene expression patternanalysis revealed a relatively high MlWRKY12 expression level in rhizomes, stems and leaf sheaths. In situhybridization analysis further demonstrated that MlWRKY12 was expressed in vascular bundle sheath,sclerenchyma and parenchyma tissues. The heterologous expression of MlWRKY12 in an atwrky12 back-ground mutant successfully rescued the phenotype of pith cell walls caused by the defect of AtWRKY12.Most strikingly, the transgenic Arabidopsis plants overexpressing MlWRKY12 exhibited early flowering.The transcript abundance of flowering related genes was measured by quantitative RT-PCR analysis,suggesting that overexpression of MlWRKY12 in Arabidopsis had a significant impact on the expres-sion level of CONSTANS (CO). Moreover, the expression levels of FLOWERING LOCUS T (FT), LFY (LEAFY),APETALA1 (AP1), CAULIFLOWER (CAL) and FRUITFULL (FUL) were upregulated in transgenic plants. Theseresults demonstrated the conserved function of MlWRKY12 existing in secondary cell wall formation ofmonocotyledonous species and implied a possible impact of MlWRKY12 on flowering control. ; WRKY proteins play crucial roles in various plant processes. An AtWRKY12 homologous gene, named M, was isolated from Miscanthus lutarioriparius. The MlWRKY12 gene encodes a WRKY transcription factor belonging to the group llc subfamily. MlWRKY12 is a nuclear protein. Gene expression pattern analysis revealed a relatively high MlWRKY12 expression level in rhizomes, stems and leaf sheaths. In situ hybridization analysis further demonstrated that MlWRKY12 was expressed in vascular bundle sheath, sclerenchyma and parenchyma tissues. The heterologous expression of MlWRKY12 in an atwrky12 background mutant successfully rescued the phenotype of pith cell walls caused by the defect of AtWRKY12. Most strikingly, the transgenic Arabidopsis plants overexpressing MlWRKY12 exhibited early flowering. The transcript abundance of flowering related genes was measured by quantitative RT-PCR analysis, suggesting that overexpression of MlWRKY12 in Arabidopsis had a significant impact on the expression level of CONSTANS (CO). Moreover, the expression levels of FLOWERING LOCUST (FT), LFY (LEAFY), APETALA1 (AP1), CAULIFLOWER (CAL) and FRUITFULL (FUL) were upregulated in transgenic plants. These results demonstrated the conserved function of MlWRKY12 existing in secondary cell wall formation of monocotyledonous species and implied a possible impact of MlWRKY12 on flowering control. (C) 2013 Elsevier Ireland Ltd. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Mlwrky12 Miscanthus Secondary Cell Wall Flowering Time |
| 学科领域 | 能源植物资源 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.plantsci.2013.07.010 |
| 关键词[WOS] | ARABIDOPSIS-THALIANA ; SINENSIS ANDERSS ; LEAF SENESCENCE ; ENERGY CROP ; SWITCHGRASS ; SIGNALS ; PLANTS ; IDENTIFICATION ; PATHWAY ; BIOMASS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS类目 | Biochemistry & Molecular Biology ; Plant Sciences |
| WOS记录号 | WOS:000326133500001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1591 |
| 专题 | 资源植物与环境工程研究组 |
| 作者单位 | Chinese Acad Sci QIBEBT CAS, Key Lab Biofuels, Inst BioEnergy & Bioproc Technol, Chinese Acad Sci, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Yanchong,Hu, Ruibo,Wang, Huamei,et al. MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering[J]. PLANT SCIENCE,2013,212(212):1-9. |
| APA | Yu, Yanchong.,Hu, Ruibo.,Wang, Huamei.,Cao, Yingping.,He, Guo.,...&Zhou, Gongke.(2013).MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering.PLANT SCIENCE,212(212),1-9. |
| MLA | Yu, Yanchong,et al."MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering".PLANT SCIENCE 212.212(2013):1-9. |
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