| Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3 | |
Qi, Guang1,2 ; Hu, Ruibo1; Yu, Li1; Chai, Guohua1; Cao, Yingping1; Zuo, Ran1; Kong, Yingzhen3; Zhou, Gongke1
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| 2013-09-15 | |
| 发表期刊 | JOURNAL OF PLANT PHYSIOLOGY
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| 卷号 | 170期号:14页码:1267-1276 |
| 摘要 | Root hairs are tip-growing long tubular outgrowths of specialized epidermal cells, and are important for nutrient and water uptake and interaction with the soil microflora. Here we characterized two poplar cellulose synthase-like D (CSLD) genes, PdCSLD5 and PdCSLD6, the most probable orthologs to the Arabidopsis AtCSLD3/KOJAK gene. Both PdCSLD5 and PdCSLD6 are strongly expressed in roots, including in the root hairs. Subcellular localization experiments showed that these two proteins are located not only in the polarized plasma membrane of root hair tips, but also in Golgi apparatus of the root hair and nonhair- forming cells. Overexpression of these two poplar genes in the atcsld3 mutant was able to rescue most of the defects caused by disruption of AtCSLD3, including root hair morphological changes, altered cell wall monosaccharide composition, increased non-crystalline _-1,4-glucan and decreased crystalline cellulose contents. Taken together, our results provide evidence indicating that PdCSLD5 and PdCSLD6 are functionally conserved with AtCSLD3 and support a role for PdCSLD5 and PdCSL6 specifically in crystalline cellulose production in poplar root hair tips. The results presented here also suggest that at least part of the mechanism of root hair formation is conserved between herbaceous and woody plants. ; Root hairs are tip-growing long tubular outgrowths of specialized epidermal cells, and are important for nutrient and water uptake and interaction with the soil microflora. Here we characterized two poplar cellulose synthase-like D (CSLD) genes, PdCSLD5 and PdCSLD6, the most probable orthologs to the Arabidopsis AtCSLD3/KOJAK gene. Both PdCSLD5 and PdCSLD6 are strongly expressed in roots, including in the root hairs. Subcellular localization experiments showed that these two proteins are located not only in the polarized plasma membrane of root hair tips, but also in Golgi apparatus of the root hair and non-hair-forming cells. Overexpression of these two poplar genes in the atcsld3 mutant was able to rescue most of the defects caused by disruption of AtCSLD3, including root hair morphological changes, altered cell wall monosaccharide composition, increased non-crystalline beta-1,4-glucan and decreased crystalline cellulose contents. Taken together, our results provide evidence indicating that PdCSLD5 and PdCSLD6 are functionally conserved with AtCSLD3 and support a role for PdCSLD5 and PdCSL6 specifically in crystalline cellulose production in poplar root hair tips. The results presented here also suggest that at least part of the mechanism of root hair formation is conserved between herbaceous and woody plants. (C) 2013 Elsevier GmbH. All rights reserved. |
| 文章类型 | Article |
| 关键词 | Cellulose Synthase-like d Crystalline Cellulose Poplar Root Hair |
| 学科领域 | 能源植物资源 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1016/j.jplph.2013.04.014 |
| 关键词[WOS] | CELL-WALL SYNTHESIS ; ROOT HAIR-GROWTH ; POPULUS-TRICHOCARPA ; HIGHER-PLANTS ; FAMILY ; THALIANA ; HOMOGALACTURONAN ; MORPHOGENESIS ; BIOSYNTHESIS ; SUPERFAMILY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Plant Sciences |
| WOS类目 | Plant Sciences |
| WOS记录号 | WOS:000324081200007 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1589 |
| 专题 | 资源植物与环境工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266107, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA |
| 推荐引用方式 GB/T 7714 | Qi, Guang,Hu, Ruibo,Yu, Li,et al. Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3[J]. JOURNAL OF PLANT PHYSIOLOGY,2013,170(14):1267-1276. |
| APA | Qi, Guang.,Hu, Ruibo.,Yu, Li.,Chai, Guohua.,Cao, Yingping.,...&Zhou, Gongke.(2013).Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3.JOURNAL OF PLANT PHYSIOLOGY,170(14),1267-1276. |
| MLA | Qi, Guang,et al."Two poplar cellulose synthase-like D genes, PdCSLD5 and PdCSLD6, are functionally conserved with Arabidopsis CSLD3".JOURNAL OF PLANT PHYSIOLOGY 170.14(2013):1267-1276. |
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