| GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage | |
| Kong, Yingzhen1; Zhou, Gongke4; Abdeen, Ashraf A.5; Schafhauser, James6; Richardson, Beth2; Atmodjo, Melani A.1; Jung, Jiyoung3; Wicker, Louise3; Mohnen, Debra1; Western, Tamara5; Hahn, Michael G.1,2 | |
| 2013-11-01 | |
| 发表期刊 | PLANT PHYSIOLOGY
 |
| 卷号 | 163期号:3页码:1203-1217 |
| 摘要 | The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; At1g02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATL5 is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATL5 is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATL5 gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgatl5-1 mutants with the wild-type AtGATL5 gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgatl5-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgatl5-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATL5 might function in the regulation of the final size of the mucilage rhamnogalacturonan I. ; The function of a putative galacturonosyltransferase from Arabidopsis (Arabidopsis thaliana; At1g02720; GALACTURONOSYLTRANSFERASE-LIKE5 [AtGATL5]) was studied using a combination of molecular genetic, chemical, and immunological approaches. AtGATL5 is expressed in all plant tissues, with highest expression levels in siliques 7 DPA. Furthermore, its expression is positively regulated by several transcription factors that are known to regulate seed coat mucilage production. AtGATL5 is localized in both endoplasmic reticulum and Golgi, in comparison with marker proteins resident to these subcellular compartments. A transfer DNA insertion in the AtGATL5 gene generates seed coat epidermal cell defects both in mucilage synthesis and cell adhesion. Transformation of atgatl5-1 mutants with the wild-type AtGATL5 gene results in the complementation of all morphological phenotypes. Compositional analyses of the mucilage isolated from the atgatl5-1 mutant demonstrated that galacturonic acid and rhamnose contents are decreased significantly in atgatl5-1 compared with wild-type mucilage. No changes in structure were observed between soluble mucilage isolated from wild-type and mutant seeds, except that the molecular weight of the mutant mucilage increased 63% compared with that of the wild type. These data provide evidence that AtGATL5 might function in the regulation of the final size of the mucilage rhamnogalacturonan I. |
| 文章类型 | Article |
| 学科领域 | 能源植物资源 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1104/pp.113.227041 |
| 关键词[WOS] | BIOSYNTHETIC HOMOGALACTURONAN GALACTURONOSYLTRANSFERASE ; CELL-ADHESION ; GENE ENCODES ; RHAMNOGALACTURONAN-I ; TRANSCRIPTION FACTOR ; XYLOGLUCAN BIOSYNTHESIS ; PECTIC POLYSACCHARIDES ; FUNCTIONAL-ANALYSIS ; CELLULOSE SYNTHESIS ; XYLAN BIOSYNTHESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Plant Sciences |
| 项目资助者 | National Science Foundation(MCB-0646109) ; Department of Agriculture(2010-65115-20396) ; Department of Energy(DE-FG09-93ER-20097) ; Natural Sciences and Engineering Council of Canada ; National Science Foundation Plant Genome Program(DCB-041683 ; IOS-0923992) |
| WOS类目 | Plant Sciences |
| WOS记录号 | WOS:000326520900011 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1582 |
| 专题 | 资源植物与环境工程研究组 |
| 作者单位 | 1.Univ Georgia, Complex Carbohydrate Res Ctr, Athens, GA 30602 USA 2.Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA 3.Univ Georgia, Dept Food Sci & Technol, Athens, GA 30602 USA 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao 266101, Peoples R China 5.McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada 6.McGill Univ, Dept Microbiol & Immunol, Montreal, PQ H3G 1A4, Canada |
| 推荐引用方式 GB/T 7714 | Kong, Yingzhen,Zhou, Gongke,Abdeen, Ashraf A.,et al. GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage[J]. PLANT PHYSIOLOGY,2013,163(3):1203-1217. |
| APA | Kong, Yingzhen.,Zhou, Gongke.,Abdeen, Ashraf A..,Schafhauser, James.,Richardson, Beth.,...&Hahn, Michael G..(2013).GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage.PLANT PHYSIOLOGY,163(3),1203-1217. |
| MLA | Kong, Yingzhen,et al."GALACTURONOSYLTRANSFERASE-LIKE5 Is Involved in the Production of Arabidopsis Seed Coat Mucilage".PLANT PHYSIOLOGY 163.3(2013):1203-1217. |
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