| An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't] | |
| Yu Ying; Dongdong Meng; Xiaohua Chen; Fuli Li | |
| 2013 | |
| 发表期刊 | Enzyme Microb Technol
 |
| 卷号 | 53期号:3页码:194-199 |
| 摘要 | An anaerobic, extremely thermophilic, and cellulose- and xylan-degrading bacterium F32 was isolated from biocompost. Sequence analysis of the 16S rRNA gene of this strain showed that it was closely related to Caldicellulosiruptor saccharolyticus DSM 8903 (99.0% identity). Physiological and biochemical data also supported that identification of strain F32 as a Caldicellulosiruptor species. The proteins secreted by Caldicellulosiruptor sp. F32 grown on xylan showed a xylanase activity of 7.74 U/mg, which was 2.5 times higher than that of C. saccharolyticus DSM 8903. Based on the genomic sequencing data, 2 xylanase genes, JX030400 and JX030401, were identified in Caldicellulosiruptor sp. F32. The xylanase encoded by JX030401 shared 97% identity with Csac 0696 of C. saccharolyticus DSM 8903, while that encoded by JX030400 shared 94% identity with Athe 0089 of C. bescii DSM 6725, which was not found in the genome of strain DSM 8903. Xylanse encoded by JX030400 had 9-fold higher specific activity than JX030401. Our results indicated that although the 2 strains shared high identity, the xylanase system in Caldicellulosiruptor sp. F32 was more efficient than that in C. saccharolyticus DSM 8903. |
| 关键词 | Lignocellulosic Biomass Caldicellulosiruptor Sp. Xylanase Hyperthermophile Anaerobe |
| 学科领域 | 微生物资源 |
| 收录类别 | SCI |
| 语种 | 英语 |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1559 |
| 专题 | 分子微生物工程研究组 |
| 推荐引用方式 GB/T 7714 | Yu Ying,Dongdong Meng,Xiaohua Chen,et al. An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't][J]. Enzyme Microb Technol,2013,53(3):194-199. |
| APA | Yu Ying,Dongdong Meng,Xiaohua Chen,&Fuli Li.(2013).An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't].Enzyme Microb Technol,53(3),194-199. |
| MLA | Yu Ying,et al."An extremely thermophilic anaerobic bacterium Caldicellulosiruptor sp. F32 exhibits distinctive properties in growth and xylanases during xylan hydrolysis. [Research Support, Non-U.S. Gov't]".Enzyme Microb Technol 53.3(2013):194-199. |
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