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Isolation and characterization of Aurantiochytrium species high docosahexaenoic acid (DHA) production by the newly isolated microalga
Mang Gao; Xiaojin Song; Yingang Feng; Wenli Li; Qiu Cui
2013
发表期刊Journal of Oleo Science
卷号62期号:3页码:143–151
摘要

Abstract: A heterotrophic microalga, strain SD116, with the ability to produce high concentrations of docosahexaenoic acid (DHA, C22:6n-3) was isolated from Shuidong Bay, Guangdong Province, China. Nucleotide sequence analysis of the 18S rDNA of SD116 showed that the strain has a close phylogenetic relationship to Aurantiochytrium species. The highest rates for growth and DHA accumulation for SD116 were obtained in 6.0% glucose, 2.0% yeast extract, and 50% artificial seawater (ASW) at a pH of 7 at 28℃. The maximum total lipid content reached 56.3% of the dry cell weight (DCW), and the maximum DHA content accounted for 50.9% of the total fatty acid (TFA) content. It was further found that urea may be a potential nitrogen source for industrial  ermentation because of its cheap price and ability to induce a relatively high biomass and lipid production capacity. Using 5 L fermenters, the DCW, total lipid content, and DHA yield were found to be 70.43 g L-1, 71.09% of the DCW, and 17.42 g L-1 (34.79% of the TFA), respectively. The results show that Aurantiochytrium sp. SD116 is a promising candidate for commercial DHA production and could be useful for the synthesis of biomass-related products.

关键词Aurantiochytrium Sp Biomass Docosahexaenoic Acid Heterotrophic Marine Algae Lipid
学科领域代谢物组学
语种英语
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/1460
专题代谢物组学研究组
推荐引用方式
GB/T 7714
Mang Gao,Xiaojin Song,Yingang Feng,et al. Isolation and characterization of Aurantiochytrium species high docosahexaenoic acid (DHA) production by the newly isolated microalga[J]. Journal of Oleo Science,2013,62(3):143–151.
APA Mang Gao,Xiaojin Song,Yingang Feng,Wenli Li,&Qiu Cui.(2013).Isolation and characterization of Aurantiochytrium species high docosahexaenoic acid (DHA) production by the newly isolated microalga.Journal of Oleo Science,62(3),143–151.
MLA Mang Gao,et al."Isolation and characterization of Aurantiochytrium species high docosahexaenoic acid (DHA) production by the newly isolated microalga".Journal of Oleo Science 62.3(2013):143–151.
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