| Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen | |
Dai, Xin1; Zhu, Yaxin1; Luo, Yingfeng2; Song, Lei1; Liu, Di3; Liu, Li3; Chen, Furong1; Wang, Min1; Li, Jiabao1; Zeng, Xiaowei4; Dong, Zhiyang1; Hu, Songnian2; Li, Lingyan1; Xu, Jian4 ; Huang, Li1; Dong, Xiuzhu1
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| 2012-07-13 | |
| 发表期刊 | PLOS ONE
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| 卷号 | 7期号:7页码:e40430 |
| 摘要 | The rumen hosts one of the most efficient microbial systems for degrading plant cell walls, yet the predominant cellulolytic proteins and fibrolytic mechanism(s) remain elusive. Here we investigated the cellulolytic microbiome of the yak rumen by using a combination of metagenome-based and bacterial artificial chromosome (BAC)-based functional screening approaches. Totally 223 fibrolytic BAC clones were pyrosequenced and 10,070 ORFs were identified. Among them 150 were annotated as the glycoside hydrolase (GH) genes for fibrolytic proteins, and the majority (69%) of them were clustered or linked with genes encoding related functions. Among the 35 fibrolytic contigs of >10 Kb in length, 25 were derived from Bacteroidetes and four from Firmicutes. Coverage analysis indicated that the fibrolytic genes on most Bacteroidetes-contigs were abundantly represented in the metagenomic sequences, and they were frequently linked with genes encoding SusC/SusD-type outer-membrane proteins. GH5, GH9, and GH10 cellulase/hemicellulase genes were predominant, but no GH48 exocellulase gene was found. Most (85%) of the cellulase and hemicellulase proteins possessed a signal peptide; only a few carried carbohydrate-binding modules, and no cellulosomal domains were detected. These findings suggest that the SucC/SucD-involving mechanism, instead of one based on cellulosomes or the free-enzyme system, serves a major role in lignocellulose degradation in yak rumen. Genes encoding an endoglucanase of a novel GH5 subfamily occurred frequently in the metagenome, and the recombinant proteins encoded by the genes displayed moderate Avicelase in addition to endoglucanase activities, suggesting their important contribution to lignocellulose degradation in the exocellulase-scarce rumen. |
| 文章类型 | Article |
| 学科领域 | 功能基因组 |
| WOS标题词 | Science & Technology |
| DOI | 10.1371/journal.pone.0040430 |
| 关键词[WOS] | OUTER-MEMBRANE PROTEINS ; BACTEROIDES-THETAIOTAOMICRON ; GLYCOSIDE HYDROLASES ; BACTERIAL ; GENOME ; GENES ; DEGRADATION ; DIVERSITY ; DISCOVERY ; CELLULOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Science & Technology - Other Topics |
| WOS类目 | Multidisciplinary Sciences |
| WOS记录号 | WOS:000306406700028 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1423 |
| 专题 | 单细胞中心组群 |
| 作者单位 | 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing, Peoples R China 2.Chinese Acad Sci, Beijing Inst Gen, CAS Key Lab Genome Sci & Informat, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Bioinformat Ctr, Beijing, Peoples R China 4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioprocess Technol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Xin,Zhu, Yaxin,Luo, Yingfeng,et al. Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen[J]. PLOS ONE,2012,7(7):e40430. |
| APA | Dai, Xin.,Zhu, Yaxin.,Luo, Yingfeng.,Song, Lei.,Liu, Di.,...&Dong, Xiuzhu.(2012).Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen.PLOS ONE,7(7),e40430. |
| MLA | Dai, Xin,et al."Metagenomic Insights into the Fibrolytic Microbiome in Yak Rumen".PLOS ONE 7.7(2012):e40430. |
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