Enhancement of engineered trifunctional enzyme by optimizing linker peptides for degradation of agricultur | |
Wang, R. L; Xue, Y. M.; Wu, X. X. Song, X. F. | |
2010 | |
发表期刊 | Enzyme Microb. Tech. |
卷号 | 47期号:1页码:194-199 |
摘要 | Fusion proteins composed of a xylosidase–arabinosidase (Xar) from Thermoanaerobacter ethanolicus andThermomyces lanuginosu xylanase (XynA) were construted using different peptidelinkers. The aim was to create optimal linkers that were able to join the functional modules without disturbing their function. Five fusion variants with linker containing different length and ratio of Ala/Gly were produced in Escherichia coliand purified for characterization of their enzymatic and kinetic properties. The fusion proteins with linkershad similar temperature and pH profiles to those without linkers, being well in accordance with their parents. However, the fusion variant containing SAGSSAAGSGSG had greater stability for xylosidase–arabinosidase activity than other fusion variants at 65–80 °C and pH 6.6–8.2. An obvious increase in activity, which occurred in the first 60 min at 65–75 °C for xylosidase–arabinosidase, appeared for xylanase and arabinosidase at pH 6.6–7.8, and for xylosidase at pH 7.8–9.5. The reducing sugar release efficiency of fusion variants containing SAGSSAAGSGSG and SGGSSAAGSGSG were obviously higher compared with those containing other linkers. These results indicate that introducing Ala in monotonous peptidesconsisting of only Gly and Ser would make the best linkers for the Xar–XynA fusion protein. |
学科领域 | 仿真与模拟 |
语种 | 英语 |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1343 |
专题 | 蛋白质设计研究组 |
推荐引用方式 GB/T 7714 | Wang, R. L,Xue, Y. M.,Wu, X. X. Song, X. F.. Enhancement of engineered trifunctional enzyme by optimizing linker peptides for degradation of agricultur[J]. Enzyme Microb. Tech.,2010,47(1):194-199. |
APA | Wang, R. L,Xue, Y. M.,&Wu, X. X. Song, X. F..(2010).Enhancement of engineered trifunctional enzyme by optimizing linker peptides for degradation of agricultur.Enzyme Microb. Tech.,47(1),194-199. |
MLA | Wang, R. L,et al."Enhancement of engineered trifunctional enzyme by optimizing linker peptides for degradation of agricultur".Enzyme Microb. Tech. 47.1(2010):194-199. |
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