蛋白质设计研究组知识库

抗坏血酸过氧化物酶(ascorbate peroxidase, APX)是植物体内活性氧清除的关键酶，在植物逆境应答中发挥着重要作用。通过构建水稻OsAPX1基因的过表达植物载体，利用根癌农杆菌介导法导入水稻品种日本晴获得转基因植株。半定量RT-PCR分析表明，转基因植株中OsAPX1表达水平明显提高。田间T0代过表达转基因植株在高温条件下的秕谷率显著低于野生型。热激胁迫实验显示，转基因株系比野生型具有更强的耐热性。在过氧化氢胁迫下，转基因植株也表现出更强的抗氧化能力。该结果表明水稻OsAPX1基因在活性氧清除中发挥功能，过量表达OsAPX1能够增强水稻的高温抗性。

Ascorbate peroxidase (APX), a key enzyme to scavenge active oxygen species in plants, plays an important role in stress responses. In the study, over-expression vector of rice OsAPX1 gene was constructed and introduced into rice cultivar Nipponbare by Agrobacterium-mediated transformation. The semi-quantitative RT-PCR analysis revealed the remarkable increase of OsAPX1 level in transgenic plants. The primary generation (T0) over-expression lines showed significant lower sterile rates in field under high temperature than that of the wild type. The transgenic plants exhibited improved resistance to heat and hydrogen peroxide. It suggested that rice OsAPX1 gene has an effect on active oxygen scavenging, and over-expression of OsAPX1 could enhance heat tolerance in rice.