Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellumstrain

doi:10.1186/1754-6834-7-75

QIBEBT-IR

	Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellumstrain
	Yee,Kelsey L 1,2; Rodriguez Jr,Miguel 1,2; Thompson,Olivia A 1,2; Fu,Chunxiang3,4 ; Wang,Zeng-Yu 2,3; Davison,Brian H 1,2; Mielenz,Jonathan R 1,2,5
	2014-05-22
发表期刊	Biotechnology for Biofuels
ISSN	1754-6834
卷号	7 期号:1
摘要	AbstractBackgroundSwitchgrass is an abundant and dedicated bioenergy feedstock, however its inherent recalcitrance is one of the economic hurdles for producing biofuels. The downregulation of the caffeic acid O-methyl transferase (COMT) gene in the lignin pathway of switchgrass reduced lignin content and S/G ratio, and the transgenic lines showed improved fermentation yield with Saccharomyces cerevisiae and wild-type Clostridium thermocellum (ATCC 27405) in comparison to the wild-type switchgrass.ResultsHere we examine the conversion and yield of the COMT transgenic and wild-type switchgrass lines with an engineered and evolved C. thermocellum (M1570) strain. The fermentation of the transgenic switchgrass by M1570 had superior conversion relative to the wild-type control switchgrass line with an increase in conversion of approximately 20% and ethanol being the primary product accounting for 90% of the total metabolites measured by HPLC analysis.ConclusionsThe engineered and evolved C. thermocellum M1570 was found to respond to the apparent reduced recalcitrance of the COMT switchgrass with no substrate inhibition, producing more ethanol on the transgenic feedstock than the wild-type substrate. Since ethanol was the main fermentation metabolite produced by an engineered and evolved C. thermocellum strain, its ethanol yield on a transgenic switchgrass substrate (gram/gram (g/g) glucan liberated) is the highest produced thus far. This result indicates that the advantages of a modified feedstock can be combined with a modified consolidated bioprocessing microorganism as anticipated.
关键词	Transgenic Switchgrass Metabolic engineering Clostridium thermocellum Consolidated bioprocessing Feedstock Cellulosic ethanol
DOI	10.1186/1754-6834-7-75
语种	英语
WOS记录号	BMC:10.1186/1754-6834-7-75
出版者	BioMed Central
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12603
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Davison,Brian H
作者单位	1.Oak Ridge National Laboratory; Biosciences Division 2.BioEnergy Science Center, Oak Ridge National Laboratory 3.The Samuel Roberts Noble Foundation; Forage Improvement Division 4.Qingdao Institute of Bioenergy and Bioprocess Technology, CAS 5.White Cliff Biosystems
推荐引用方式 GB/T 7714	Yee,Kelsey L,Rodriguez Jr,Miguel,Thompson,Olivia A,et al. Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellumstrain[J]. Biotechnology for Biofuels,2014,7(1).
APA	Yee,Kelsey L.,Rodriguez Jr,Miguel.,Thompson,Olivia A.,Fu,Chunxiang.,Wang,Zeng-Yu.,...&Mielenz,Jonathan R.(2014).Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellumstrain.Biotechnology for Biofuels,7(1).
MLA	Yee,Kelsey L,et al."Consolidated bioprocessing of transgenic switchgrass by an engineered and evolved Clostridium thermocellumstrain".Biotechnology for Biofuels 7.1(2014).