De-novo synthesis of 2-phenylethanol by Enterobactersp. CGMCC 5087

doi:10.1186/1472-6750-14-30

QIBEBT-IR

	De-novo synthesis of 2-phenylethanol by Enterobactersp. CGMCC 5087
	Zhang,Haibo1 ; Cao,Mingle 2; Jiang,Xinglin 1; Zou,Huibin 1; Wang,Cong1 ; Xu,Xin1 ; Xian,Mo1
	2014-04-25
发表期刊	BMC Biotechnology
ISSN	1472-6750
卷号	14 期号:1
摘要	AbstractBackground2-phenylethanl (2-PE) and its derivatives are important chemicals, which are widely used in food materials and fine chemical industries and polymers and it’s also a potentially valuable alcohol for next-generation biofuel. However, the biosynthesis of 2-PE are mainly biotransformed from phenylalanine, the price of which barred the production. Therefore, it is necessary to seek more sustainable technologies for 2-PE production.ResultsA new strain which produces 2-PE through the phenylpyruvate pathway was isolated and identified as Enterobacter sp. CGMCC 5087. The strain is able to use renewable monosaccharide as the carbon source and NH4Cl as the nitrogen source to produce 2-PE. Two genes of rate-limiting enzymes, chorismate mutase p-prephenate dehydratase (PheA) and 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase (DAHP), were cloned from Escherichia coli and overexpressed in E. sp. CGMCC 5087. The engineered E. sp. CGMCC 5087 produces 334.9?mg?L-1 2-PE in 12?h, which is 3.26 times as high as the wild strain.ConclusionsThe phenylpyruvate pathway and the substrate specificity of 2-keto-acid decarboxylase towards phenylpyruvate were found in E. sp. CGMCC 5087. Combined with the low-cost monosaccharide as the substrate, the finding provides a novel and potential way for 2-PE production.
关键词	2-Phenylethanol Biosynthesis Ehrlich pathway Enterobacter sp
DOI	10.1186/1472-6750-14-30
语种	英语
WOS记录号	BMC:10.1186/1472-6750-14-30
出版者	BioMed Central
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12562
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Xian,Mo
作者单位	1.CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences 2.University of Marburg
推荐引用方式 GB/T 7714	Zhang,Haibo,Cao,Mingle,Jiang,Xinglin,et al. De-novo synthesis of 2-phenylethanol by Enterobactersp. CGMCC 5087[J]. BMC Biotechnology,2014,14(1).
APA	Zhang,Haibo.,Cao,Mingle.,Jiang,Xinglin.,Zou,Huibin.,Wang,Cong.,...&Xian,Mo.(2014).De-novo synthesis of 2-phenylethanol by Enterobactersp. CGMCC 5087.BMC Biotechnology,14(1).
MLA	Zhang,Haibo,et al."De-novo synthesis of 2-phenylethanol by Enterobactersp. CGMCC 5087".BMC Biotechnology 14.1(2014).