QIBEBT-IR
OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP
Wei, Li1; Xu, Jian1
2018-06-01
Source PublicationJOURNAL OF PHYCOLOGY
ISSN0022-3646
Volume54Issue:3Pages:358-367
AbstractEpigenetic factors such as histone modifications play integral roles in plant development and stress response, yet their implications in algae remainpoorly understood. In the industrial oleaginousmicroalgae Nannochloropsis spp., the lack of an efficient methodology for chromatin immunoprecipitation (ChIP), which determines the specific genomic location of various histone modifications, has hindered probing the epigenetic basis of their photosynthetic carbon conversion and storage as oil. Here, a detailed ChIP protocol was developed for Nannochloropsis oceanica, which represents a reliable approach for the analysis of histone modifications, chromatin state, and transcription factor-binding sites at the epigenetic level. Using ChIP-qPCR, genes related to photosynthetic carbon fixation in this microalga were systematically assessed. Furthermore, a ChIP-Seq protocol was established and optimized, which generated a genome-wide profile of histone modification events, using histone mark H3K9Ac as an example. These results are the first step for appreciation of the chromatin landscape in industrial oleaginous microalgae and for epigenetics-based microalgal feedstock development.
KeywordChIP-qPCR ChIP-Seq chromatin immunoprecipitation epigenomics histone modifications Nannochloropsis oceanica
DOI10.1111/jpy.12623
WOS KeywordOLEAGINOUS MICROALGAE ; LIPID PRODUCTION ; TRIACYLGLYCEROL BIOSYNTHESIS ; ALGA NANNOCHLOROPSIS ; OCEANICA CCMP1779 ; GADITANA ; MODEL ; ACYLTRANSFERASES ; OVEREXPRESSION ; MECHANISMS
Language英语
Funding ProjectNational Natural Science Foundation of China[31741005] ; Biological Carbon Sequestration Program from Chinese Academy of Sciences[ZDRW-ZS-2016-3] ; Biological Carbon Sequestration Program from Chinese Academy of Sciences[KSZD-EW-Z-017]
WOS Research AreaPlant Sciences ; Marine & Freshwater Biology
Funding OrganizationNational Natural Science Foundation of China ; Biological Carbon Sequestration Program from Chinese Academy of Sciences
WOS SubjectPlant Sciences ; Marine & Freshwater Biology
WOS IDWOS:000435798700005
PublisherWILEY
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/12508
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorWei, Li; Xu, Jian
Affiliation1.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wei, Li,Xu, Jian. OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP[J]. JOURNAL OF PHYCOLOGY,2018,54(3):358-367.
APA Wei, Li,&Xu, Jian.(2018).OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP.JOURNAL OF PHYCOLOGY,54(3),358-367.
MLA Wei, Li,et al."OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP".JOURNAL OF PHYCOLOGY 54.3(2018):358-367.
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