QIBEBT-IR
OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP
Wei, Li1; Xu, Jian1
2018-06-01
发表期刊JOURNAL OF PHYCOLOGY
ISSN0022-3646
卷号54期号:3页码:358-367
摘要Epigenetic factors such as histone modifications play integral roles in plant development and stress response, yet their implications in algae remainpoorly understood. In the industrial oleaginousmicroalgae Nannochloropsis spp., the lack of an efficient methodology for chromatin immunoprecipitation (ChIP), which determines the specific genomic location of various histone modifications, has hindered probing the epigenetic basis of their photosynthetic carbon conversion and storage as oil. Here, a detailed ChIP protocol was developed for Nannochloropsis oceanica, which represents a reliable approach for the analysis of histone modifications, chromatin state, and transcription factor-binding sites at the epigenetic level. Using ChIP-qPCR, genes related to photosynthetic carbon fixation in this microalga were systematically assessed. Furthermore, a ChIP-Seq protocol was established and optimized, which generated a genome-wide profile of histone modification events, using histone mark H3K9Ac as an example. These results are the first step for appreciation of the chromatin landscape in industrial oleaginous microalgae and for epigenetics-based microalgal feedstock development.
关键词ChIP-qPCR ChIP-Seq chromatin immunoprecipitation epigenomics histone modifications Nannochloropsis oceanica
DOI10.1111/jpy.12623
关键词[WOS]OLEAGINOUS MICROALGAE ; LIPID PRODUCTION ; TRIACYLGLYCEROL BIOSYNTHESIS ; ALGA NANNOCHLOROPSIS ; OCEANICA CCMP1779 ; GADITANA ; MODEL ; ACYLTRANSFERASES ; OVEREXPRESSION ; MECHANISMS
语种英语
资助项目National Natural Science Foundation of China[31741005] ; Biological Carbon Sequestration Program from Chinese Academy of Sciences[ZDRW-ZS-2016-3] ; Biological Carbon Sequestration Program from Chinese Academy of Sciences[KSZD-EW-Z-017]
WOS研究方向Plant Sciences ; Marine & Freshwater Biology
项目资助者National Natural Science Foundation of China ; Biological Carbon Sequestration Program from Chinese Academy of Sciences
WOS类目Plant Sciences ; Marine & Freshwater Biology
WOS记录号WOS:000435798700005
出版者WILEY
引用统计
文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/12508
专题中国科学院青岛生物能源与过程研究所
通讯作者Wei, Li; Xu, Jian
作者单位1.Chinese Acad Sci, Single Cell Ctr, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Shandong Key Lab Energy Genet, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
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Wei, Li,Xu, Jian. OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP[J]. JOURNAL OF PHYCOLOGY,2018,54(3):358-367.
APA Wei, Li,&Xu, Jian.(2018).OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP.JOURNAL OF PHYCOLOGY,54(3),358-367.
MLA Wei, Li,et al."OPTIMIZED METHODS OF CHROMATIN IMMUNOPRECIPITATION FOR PROFILING HISTONE MODIFICATIONS IN INDUSTRIAL MICROALGAE NANNOCHLOROPSIS SPP".JOURNAL OF PHYCOLOGY 54.3(2018):358-367.
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