| Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003 | |
Wang, Shu1,4; Mao, Xiangzhao2; Wang, Hualei3; Lin, Jinping3; Li, Fuli1 ; Wei, Dongzhi3
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| 2011-07-01 | |
| 发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| 卷号 | 91期号:2页码:287-294 |
| 摘要 | A novel water-soluble dextran was synthesized from maltodextrin by cell-free extract of Gluconobacter oxydans DSM 2003. The dextran was purified by size exclusion chromatography, and the structure was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and gas chromatography-mass spectrometer. Based on the spectral data, we found that the dextran contained only D-glucose residues. The ratio of nonreducing end glucopyranosyl (Glcp) to 6-linked Glcp to 4,6-linked Glcp was estimated to be 8.62:78.79:12.59 by methylation analysis. This result indicated the existence of a small proportion of α(1,4) branches in α(1,6) glucosyl linear chains. Here, we reported the first time a novel dextran was synthesized by G. oxydans DSM 2003.; A novel water-soluble dextran was synthesized from maltodextrin by cell-free extract of Gluconobacter oxydans DSM 2003. The dextran was purified by size exclusion chromatography, and the structure was determined by Fourier transform infrared spectroscopy, nuclear magnetic resonance, and gas chromatography-mass spectrometer. Based on the spectral data, we found that the dextran contained only d-glucose residues. The ratio of nonreducing end glucopyranosyl (Glcp) to 6-linked Glcp to 4,6-linked Glcp was estimated to be 8.62:78.79:12.59 by methylation analysis. This result indicated the existence of a small proportion of alpha(1,4) branches in alpha(1,6) glucosyl linear chains. Here, we reported the first time a novel dextran was synthesized by G. oxydans DSM 2003. |
| 文章类型 | Article |
| 关键词 | Dextran Gluconobacter Oxydans Monosaccharide Composition Structure Analysis |
| 学科领域 | 微生物资源 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s00253-011-3267-6 |
| 关键词[WOS] | MAGNETIC-RESONANCE SPECTROSCOPY ; METHYLATION STRUCTURAL-ANALYSIS ; NMR-SPECTROSCOPY ; POLYSACCHARIDES ; OLIGOSACCHARIDES ; DEXTRINASE ; GLUCAN ; IR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000292203200006 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1242 |
| 专题 | 分子微生物工程研究组 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China 3.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shu,Mao, Xiangzhao,Wang, Hualei,et al. Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,91(2):287-294. |
| APA | Wang, Shu,Mao, Xiangzhao,Wang, Hualei,Lin, Jinping,Li, Fuli,&Wei, Dongzhi.(2011).Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,91(2),287-294. |
| MLA | Wang, Shu,et al."Characterization of a novel dextran produced by Gluconobacter oxydans DSM 2003".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 91.2(2011):287-294. |
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