| Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing | |
| Hu, Nan1,2; Yuan, Bo2; Sun, Juan1; Wang, Shi-An2; Li, Fu-Li2 | |
| 2012-09-01 | |
| 发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 卷号 | 95期号:5页码:1359-1368 |
| 摘要 | Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidated bioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and 65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin and Jerusalem artichoke tuber flour at 40 °C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highest ethanol concentration in Jerusalem artichoke tuber flour fermentation (200 g L(-1)) at 40 °C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 g L(-1), which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 °C, temperature did not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiority of K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It is proposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP.; Thermotolerant inulin-utilizing yeast strains are desirable for ethanol production from Jerusalem artichoke tubers by consolidated bioprocessing (CBP). To obtain such strains, 21 naturally occurring yeast strains isolated by using an enrichment method and 65 previously isolated Saccharomyces cerevisiae strains were investigated in inulin utilization, extracellular inulinase activity, and ethanol fermentation from inulin and Jerusalem artichoke tuber flour at 40 A degrees C. The strains Kluyveromyces marxianus PT-1 (CGMCC AS2.4515) and S. cerevisiae JZ1C (CGMCC AS2.3878) presented the highest extracellular inulinase activity and ethanol yield in this study. The highest ethanol concentration in Jerusalem artichoke tuber flour fermentation (200 g L-1) at 40 A degrees C achieved by K. marxianus PT-1 and S. cerevisiae JZ1C was 73.6 and 65.2 g L-1, which corresponded to the theoretical ethanol yield of 90.0 and 79.7 %, respectively. In the range of 30 to 40 A degrees C, temperature did not have a significant effect on ethanol production for both strains. This study displayed the distinctive superiority of K. marxianus PT-1 and S. cerevisiae JZ1C in the thermotolerance and utilization of inulin-type oligosaccharides reserved in Jerusalem artichoke tubers. It is proposed that both K. marxianus and S. cerevisiae have considerable potential in ethanol production from Jerusalem artichoke tubers by a high temperature CBP. |
| 文章类型 | Article |
| 关键词 | Bioethanol Consolidated Bioprocessing (Cbp) Jerusalem Artichoke Kluyveromyces Marxianus Saccharomyces Cerevisiae Thermotolerance |
| 学科领域 | 微生物资源 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s00253-012-4240-8 |
| 关键词[WOS] | YEAST PICHIA-GUILLIERMONDII ; CONTINUOUS ETHANOL-PRODUCTION ; INULIN HYDROLYSIS ; HIGH-TEMPERATURE ; EXTRACELLULAR INULINASE ; INDUSTRIAL-APPLICATIONS ; IMMOBILIZED CELLS ; ZYMOMONAS-MOBILIS ; SP-NOV. ; FERMENTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000307509800024 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1238 |
| 专题 | 分子微生物工程研究组 |
| 作者单位 | 1.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao 266109, Shandong, Peoples R China 2.Chinese Acad Sci, Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Nan,Yuan, Bo,Sun, Juan,et al. Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2012,95(5):1359-1368. |
| APA | Hu, Nan,Yuan, Bo,Sun, Juan,Wang, Shi-An,&Li, Fu-Li.(2012).Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,95(5),1359-1368. |
| MLA | Hu, Nan,et al."Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 95.5(2012):1359-1368. |
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