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Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores
Liu, Aihua1,2; Zhao, Qitao1; Krishantha, D. M. Milan1; Guan, Xiyun1
2011-06-16
Source PublicationJOURNAL OF PHYSICAL CHEMISTRY LETTERS
Volume2Issue:12Pages:1372-1376
Abstract
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The biological protein α-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the α-hemolysin pore has for a long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the α-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is dependent on the length of the duplex, and also varies with the nucleotide base composition, the modified protein pore approach offers the potential for rapid double-stranded DNA analysis, including sequencing.

; The biological protein a.-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the alpha-hemolysin pore has for a long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the alpha-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is dependent on the length of the duplex, and also varies with the nucleotide base composition, the modified protein pore approach offers the potential for rapid double-stranded DNA analysis, including sequencing.
SubtypeArticle
KeywordΑ-hemolysin Protein Engineering Double-stranded Dna Nanopore Sequencing Facilitated Dna Duplex Unzipping
Subject Area生物传感器技术
WOS HeadingsScience & Technology ; Physical Sciences ; Technology
DOI10.1021/jz200525v
WOS KeywordSTOCHASTIC DETECTION ; POLYNUCLEOTIDE MOLECULES ; PROTEIN PORES ; NANOPORE ; TRANSLOCATION ; DISCRIMINATION ; ANALYTES
Indexed BySCI
Language英语
WOS Research AreaChemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS SubjectChemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Atomic, Molecular & Chemical
WOS IDWOS:000291781700005
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Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/1215
Collection生物传感技术团队(过去)
Affiliation1.Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Nanobioelect & Biosensors, Qingdao 266101, Peoples R China
Recommended Citation
GB/T 7714
Liu, Aihua,Zhao, Qitao,Krishantha, D. M. Milan,et al. Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2011,2(12):1372-1376.
APA Liu, Aihua,Zhao, Qitao,Krishantha, D. M. Milan,&Guan, Xiyun.(2011).Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2(12),1372-1376.
MLA Liu, Aihua,et al."Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 2.12(2011):1372-1376.
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