| Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores | |
| Liu, Aihua1,2; Zhao, Qitao1; Krishantha, D. M. Milan1; Guan, Xiyun1 | |
| 2011-06-16 | |
| 发表期刊 | JOURNAL OF PHYSICAL CHEMISTRY LETTERS
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| 卷号 | 2期号:12页码:1372-1376 |
| 摘要 |  The biological protein α-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the α-hemolysin pore has for a long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the α-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is dependent on the length of the duplex, and also varies with the nucleotide base composition, the modified protein pore approach offers the potential for rapid double-stranded DNA analysis, including sequencing. ; The biological protein a.-hemolysin nanopore is under intense investigation as a potential platform for rapid and low-cost DNA sequencing. However, due to its narrow constriction, analysis of DNA in the alpha-hemolysin pore has for a long time been restricted to single strands. In this paper, we report that by introducing new surface functional groups into the alpha-hemolysin pore, facilitated unzipping of double-stranded DNA through the channel could be achieved. Since the mean residence time of the DNA events is dependent on the length of the duplex, and also varies with the nucleotide base composition, the modified protein pore approach offers the potential for rapid double-stranded DNA analysis, including sequencing. |
| 文章类型 | Article |
| 关键词 | Α-hemolysin Protein Engineering Double-stranded Dna Nanopore Sequencing Facilitated Dna Duplex Unzipping |
| 学科领域 | 生物传感器技术 |
| WOS标题词 | Science & Technology ; Physical Sciences ; Technology |
| DOI | 10.1021/jz200525v |
| 关键词[WOS] | STOCHASTIC DETECTION ; POLYNUCLEOTIDE MOLECULES ; PROTEIN PORES ; NANOPORE ; TRANSLOCATION ; DISCRIMINATION ; ANALYTES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| WOS类目 | Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Atomic, Molecular & Chemical |
| WOS记录号 | WOS:000291781700005 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1215 |
| 专题 | 生物传感技术团队（过去） |
| 作者单位 | 1.Univ Texas Arlington, Dept Chem & Biochem, Arlington, TX 76019 USA 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Nanobioelect & Biosensors, Qingdao 266101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Aihua,Zhao, Qitao,Krishantha, D. M. Milan,et al. Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2011,2(12):1372-1376. |
| APA | Liu, Aihua,Zhao, Qitao,Krishantha, D. M. Milan,&Guan, Xiyun.(2011).Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2(12),1372-1376. |
| MLA | Liu, Aihua,et al."Unzipping of Double-Stranded DNA in Engineered alpha-Hemolysin Pores".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 2.12(2011):1372-1376. |
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