| Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection | |
| Liu, Aihua1,2,3; Wu, Liyou1; He, Zhili1; Zhou, Jizhong1 | |
| 2011-10-01 | |
| 发表期刊 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 卷号 | 401期号:6页码:2003-2011 |
| 摘要 | Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-functionalized silica nanoparticles was developed, in which Alexa Fluor 647 (AF647) molecules were covalently embedded. The coating of bovine serum albumin on the resultant fluorescent particles can greatly eliminate nonspecific background signal interference. The thus-synthesized fluorescent nanoparticles can specifically recognize biotin-labeled target DNA hybridized to the microarray via streptavidin-biotin interaction. The response of this DNA microarray technology exhibited a linear range within 0.2 to 10 pM complementary DNA and limit of detection of 0.1 pM, enhancing microarray hybridization sensitivity over tenfold. This promising technology may be potentially applied to other binding events such as specific interactions between proteins.; Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-functionalized silica nanoparticles was developed, in which Alexa Fluor 647 (AF647) molecules were covalently embedded. The coating of bovine serum albumin on the resultant fluorescent particles can greatly eliminate nonspecific background signal interference. The thus-synthesized fluorescent nanoparticles can specifically recognize biotin-labeled target DNA hybridized to the microarray via streptavidin-biotin interaction. The response of this DNA microarray technology exhibited a linear range within 0.2 to 10 pM complementary DNA and limit of detection of 0.1 pM, enhancing microarray hybridization sensitivity over tenfold. This promising technology may be potentially applied to other binding events such as specific interactions between proteins. |
| 文章类型 | Article |
| 关键词 | Dna Microarray Sensitivity Silica Nanoparticles Chemically Doped Dye Biotin-streptavidin Interaction Reverse Microemulsion Reaction |
| 学科领域 | 生物传感器技术 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1007/s00216-011-5258-y |
| 关键词[WOS] | SINGLE-NUCLEOTIDE POLYMORPHISM ; RESONANCE ENERGY-TRANSFER ; REVERSE MICROEMULSION ; GOLD NANOPARTICLES ; URIC-ACID ; BIOSENSORS ; NANOTUBES ; GEOCHIP ; PROBES ; HYBRIDIZATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| WOS类目 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000295510100027 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1214 |
| 专题 | 生物传感技术团队（过去） |
| 作者单位 | 1.Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73019 USA 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Aihua,Wu, Liyou,He, Zhili,et al. Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,401(6):2003-2011. |
| APA | Liu, Aihua,Wu, Liyou,He, Zhili,&Zhou, Jizhong.(2011).Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,401(6),2003-2011. |
| MLA | Liu, Aihua,et al."Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 401.6(2011):2003-2011. |
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