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Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection
Liu, Aihua1,2,3; Wu, Liyou1; He, Zhili1; Zhou, Jizhong1
2011-10-01
发表期刊ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷号401期号:6页码:2003-2011
摘要Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-functionalized silica nanoparticles was developed, in which Alexa Fluor 647 (AF647) molecules were covalently embedded. The coating of bovine serum albumin on the resultant fluorescent particles can greatly eliminate nonspecific background signal interference. The thus-synthesized fluorescent nanoparticles can specifically recognize biotin-labeled target DNA hybridized to the microarray via streptavidin-biotin interaction. The response of this DNA microarray technology exhibited a linear range within 0.2 to 10 pM complementary DNA and limit of detection of 0.1 pM, enhancing microarray hybridization sensitivity over tenfold. This promising technology may be potentially applied to other binding events such as specific interactions between proteins.; Increasing the sensitivity in DNA microarray hybridization can significantly enhance the capability of microarray technology for a wide range of research and clinical diagnostic applications, especially for those with limited sample biomass. To address this issue, using reverse microemulsion method and surface chemistry, a novel class of homogenous, photostable, highly fluorescent streptavidin-functionalized silica nanoparticles was developed, in which Alexa Fluor 647 (AF647) molecules were covalently embedded. The coating of bovine serum albumin on the resultant fluorescent particles can greatly eliminate nonspecific background signal interference. The thus-synthesized fluorescent nanoparticles can specifically recognize biotin-labeled target DNA hybridized to the microarray via streptavidin-biotin interaction. The response of this DNA microarray technology exhibited a linear range within 0.2 to 10 pM complementary DNA and limit of detection of 0.1 pM, enhancing microarray hybridization sensitivity over tenfold. This promising technology may be potentially applied to other binding events such as specific interactions between proteins.
文章类型Article
关键词Dna Microarray Sensitivity Silica Nanoparticles Chemically Doped Dye Biotin-streptavidin Interaction Reverse Microemulsion Reaction
学科领域生物传感器技术
WOS标题词Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI10.1007/s00216-011-5258-y
关键词[WOS]SINGLE-NUCLEOTIDE POLYMORPHISM ; RESONANCE ENERGY-TRANSFER ; REVERSE MICROEMULSION ; GOLD NANOPARTICLES ; URIC-ACID ; BIOSENSORS ; NANOTUBES ; GEOCHIP ; PROBES ; HYBRIDIZATION
收录类别SCI
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000295510100027
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文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/1214
专题生物传感技术团队(过去)
作者单位1.Univ Oklahoma, Dept Bot & Microbiol, Inst Environm Genom, Norman, OK 73019 USA
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Shandong, Peoples R China
3.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Shandong, Peoples R China
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Liu, Aihua,Wu, Liyou,He, Zhili,et al. Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2011,401(6):2003-2011.
APA Liu, Aihua,Wu, Liyou,He, Zhili,&Zhou, Jizhong.(2011).Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,401(6),2003-2011.
MLA Liu, Aihua,et al."Development of highly fluorescent silica nanoparticles chemically doped with organic dye for sensitive DNA microarray detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 401.6(2011):2003-2011.
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