| A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode | |
Li, Liang2,3,4; Liang, Bo2,3 ; Shi, Jianguo5; Li, Feng4; Mascini, Marco1; Liu, Aihua2,3
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| 2012-03-15 | |
| 发表期刊 | BIOSENSORS & BIOELECTRONICS
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| 卷号 | 33期号:1页码:100-105 |
| 摘要 | A novel Nafion/bacteria-displaying xylose dehydrogenase (XDH)/multi-walled carbon nanotubes (MWNTs) composite film-modified electrode was fabricated and applied for the sensitive and selective determination ofd-xylose (INS 967), where the XDH-displayed bacteria (XDH-bacteria) was prepared using a newly identified ice nucleation protein from Pseudomonas borealis DL7 as an anchoring motif. The XDH-displayed bacteria can be used directly, eliminating further enzyme-extraction and purification, thus greatly improved the stability of the enzyme. The optimal conditions for the construction of biosensor were established: homogeneous Nafion–MWNTs composite dispersion (10 μL) was cast onto the inverted glassy carbon electrode, followed by casting 10-μL of XDH-bacteria aqueous solution to stand overnight to dry, then a 5-μL of Nafion solution (0.05 wt%) is syringed to the electrode surface. The bacteria-displaying XDH could catalyze the oxidization of xylose to xylonolactone with coenzyme NAD+ in 0.1 M PBS buffer (pH7.4), where NAD+ (nicotinamide adenine dinucleotide) is reduced to NADH (the reduced form of nicotinamide adenine dinucleotide). The resultant NADH is further electrocatalytically oxidized by MWNTs on the electrode, resulting in an obvious oxidation peak around 0.50 V (vs. Ag/AgCl). In contrast, the bacteria-XDH-only modified electrode showed oxidation peak at higher potential of 0.7 V and less sensitivity. Therefore, the electrode/MWNTs/bacteria-XDH/Nafion exhibited good analytical performance such as long-term stability, a wide dynamic range of 0.6–100 μM and a low detection limit of 0.5 μM d-xylose (S/N = 3). No interference was observed in the presence of 300-fold excess of other saccharides including d-glucose, d-fructose, d-maltose, d-galactose, d-mannose, d-sucrose, and d-cellbiose as well as 60-fold excess of l-arabinose. The proposed microbial biosensor is stable, specific, sensitive, reproducible, simple, rapid and cost-effective, which holds great potential in real applications.; A novel Nafion/bacteria-displaying xylose dehydrogenase (XDH)/multi-walled carbon nanotubes (MwNTs) composite film-modified electrode was fabricated and applied for the sensitive and selective determination of D-xylose (INS 967), where the XDH-displayed bacteria (XDH-bacteria) was prepared using a newly identified ice nucleation protein from Pseudomonas borealis DL7 as an anchoring motif. The XDH-displayed bacteria can be used directly, eliminating further enzyme-extraction and purification, thus greatly improved the stability of the enzyme. The optimal conditions for the construction of biosensor were established: homogeneous Nafion-MWNTs composite dispersion (10 mu L) was cast onto the inverted glassy carbon electrode, followed by casting 10-mu L of XDH-bacteria aqueous solution to stand overnight to dry, then a 5-mu L of Nafion solution (0.05 wt%) is syringed to the electrode surface. The bacteria-displaying XDH could catalyze the oxidization of xylose to xylonolactone with coenzyme NAD(+) in 0.1 M PBS buffer (pH7.4), where NAD(+) (nicotinamide adenine dinucleotide) is reduced to NADH (the reduced form of nicotinamide adenine dinucleotide). The resultant NADH is further electrocatalytically oxidized by MWNTs on the electrode, resulting in an obvious oxidation peak around 0.50 V (vs. Ag/AgCl). In contrast, the bacteria-XDH-only modified electrode showed oxidation peak at higher potential of 0.7 V and less sensitivity. Therefore, the electrode/MWNTs/bacteria-XDH/Nafion exhibited good analytical performance such as long-term stability, a wide dynamic range of 0.6-100 mu M and a low detection limit of 0.5 mu M D-xylose (S/N = 3). No interference was observed in the presence of 300-fold excess of other saccharides including D-glucose, D-fructose, D-maltose, D-galactose, D-mannose, is-sucrose, and D-cellbiose as well as 60-fold excess of L-arabinose. The proposed microbial biosensor is stable, specific, sensitive, reproducible, simple, rapid and cost-effective, which holds great potential in real applications. (C) 2012 Elsevier B.V. All rights reserved. |
| 文章类型 | Article |
| 关键词 | D-xylose Electrochemical Biosensor Xylose Dehydrogenase Displayed Bacteria Multi-walled Carbon Nanotubes |
| 学科领域 | 生物传感器技术 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| DOI | 10.1016/j.bios.2011.12.027 |
| 关键词[WOS] | CAPILLARY-ZONE-ELECTROPHORESIS ; DEPENDENT ALDOSE DEHYDROGENASE ; AMPEROMETRIC ENZYME ELECTRODE ; MOLECULAR-WEIGHT SACCHARIDES ; ICE NUCLEATION PROTEIN ; OLIGOSACCHARIDE DEHYDROGENASE ; GLUCONOBACTER-OXYDANS ; LIQUID-CHROMATOGRAPHY ; ALPHA-AMYLASE ; GLUCOSE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| WOS类目 | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology |
| WOS记录号 | WOS:000301699800016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1212 |
| 专题 | 生物传感技术团队（过去） |
| 作者单位 | 1.Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, Italy 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 4.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Peoples R China 5.Shandong Acad Sci, Inst Biol, Key Lab Biosensors Shangdong Prov, Jinan 250014, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Liang,Liang, Bo,Shi, Jianguo,et al. A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode[J]. BIOSENSORS & BIOELECTRONICS,2012,33(1):100-105. |
| APA | Li, Liang,Liang, Bo,Shi, Jianguo,Li, Feng,Mascini, Marco,&Liu, Aihua.(2012).A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode.BIOSENSORS & BIOELECTRONICS,33(1),100-105. |
| MLA | Li, Liang,et al."A selective and sensitive D-xylose electrochemical biosensor based on xylose dehydrogenase displayed on the surface of bacteria and multi-walled carbon nanotubes modified electrode".BIOSENSORS & BIOELECTRONICS 33.1(2012):100-105. |
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