Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas

doi:10.1186/s12864-018-4996-z

QIBEBT-IR

	Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas
	Li, Chunyan 1,2,3,5,6; Wang, Jinpeng 1,2,3,5,6; Song, Kai 1,3,5,6; Meng, Jie 1,3,5,6; Xu, Fei 1,3,5,6; Li, Li 1,4,5,6; Zhang, Guofan 1,3,5,6
	2018-08-22
发表期刊	BMC GENOMICS
ISSN	1471-2164
卷号	19 页码:15
摘要	Background: Both growth and nutritional traits are important economic traits of Crassostrea gigas (C. gigas) in industry. But few work has been done to study the genetic architecture of nutritional traits of the oyster. In this study, we constructed a high-density genetic map of C. gigas to help assemble the genome sequence onto chromosomes, meanwhile explore the genetic basis for nutritional traits via quantitative trait loci (QTL) mapping. Results: The constructed genetic map contained 5024 evenly distributed markers, with an average marker interval of 0.68 cM, thus representing the densest genetic map produced for the oyster. According to the high collinearity between the consensus map and the oyster genome, 1574 scaffold (about 70%) of the genome sequence of C. gigas were successfully anchored to 10 linkage groups (LGs) of the consensus map. Using this high-qualified genetic map, we then conducted QTL analysis for growth and nutritional traits, the latter of which includes glycogen, amino acid (AA), and fatty acid (FA). Overall, 41 QTLs were detected for 17 traits. In addition, six candidate genes identified in the QTL interval showed significant correlation with the traits on transcriptional levels. These genes include growth-related genes AMY and BMP1, AA metabolism related genes PLSCR and GR, and FA metabolism regulation genes DYRK and ADAMTS. Conclusion: Using the constructed high-qualified linkage map, this study not only assembled nearly 70% of the oyster genome sequence onto chromosomes, but also identified valuable markers and candidate genes for growth and nutritional traits, especially for AA and FA that undergone few studies before. These findings will facilitate genome assembly and molecular breeding of important economic traits in C. gigas.
关键词	Crassostrea gigas Genetic map Collinearity Quantitative trait loci (QTL) mapping Growth Nutrition
DOI	10.1186/s12864-018-4996-z
关键词[WOS]	SINGLE-NUCLEOTIDE POLYMORPHISM ; PACIFIC OYSTER ; LINKAGE MAPS ; DISEASE-RESISTANCE ; MOLECULAR-CLONING ; SUMMER MORTALITY ; VIRGINICA GMELIN ; SPERM MOTILITY ; EASTERN OYSTER ; SLC3A1 GENE
语种	英语
资助项目	National Natural Science Foundation of China[31530079] ; National Natural Science Foundation of China[31572620] ; Strategic Priority Research Program of "Western Pacific Ocean System: Structure, Dynamics and Consequences"[XDA586 11020305] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS研究方向	Biotechnology & Applied Microbiology ; Genetics & Heredity
项目资助者	National Natural Science Foundation of China ; Strategic Priority Research Program of "Western Pacific Ocean System: Structure, Dynamics and Consequences" ; Earmarked Fund for Modern Agro-industry Technology Research System
WOS类目	Biotechnology & Applied Microbiology ; Genetics & Heredity
WOS记录号	WOS:000442532000005
出版者	BMC
引用统计	被引频次：40[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12082
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Li, Li; Zhang, Guofan
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Li, Chunyan,Wang, Jinpeng,Song, Kai,et al. Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas[J]. BMC GENOMICS,2018,19:15.
APA	Li, Chunyan.,Wang, Jinpeng.,Song, Kai.,Meng, Jie.,Xu, Fei.,...&Zhang, Guofan.(2018).Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas.BMC GENOMICS,19,15.
MLA	Li, Chunyan,et al."Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas".BMC GENOMICS 19(2018):15.