Fengycins, Cyclic Lipopeptides from Marine Bacillus subtilis Strains, Kill the Plant-Pathogenic Fungus Magnaporthe grisea by Inducing Reactive Oxygen Species Production and Chromatin Condensation

doi:10.1128/AEM.00445-18

QIBEBT-IR

	Fengycins, Cyclic Lipopeptides from Marine Bacillus subtilis Strains, Kill the Plant-Pathogenic Fungus Magnaporthe grisea by Inducing Reactive Oxygen Species Production and Chromatin Condensation
	Zhang, Linlin 1,2,3,4; Sun, Chaomin 1,2,4
	2018-09-01
发表期刊	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN	0099-2240
卷号	84 期号:18 页码:17
摘要	Rice blast caused by the phytopathogen Magnaporthe grisea poses a serious threat to global food security and is difficult to control. Bacillus species have been extensively explored for the biological control of many fungal diseases. In the present study, the marine bacterium Bacillus subtilis BS155 showed a strong antifungal activity against M. grisea. The active metabolites were isolated and identified as cyclic lipopeptides (CLPs) of the fengycin family, named fengycin BS155, by the combination of high-performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry (ESI-MS/MS). Analyses using scanning and transmission electron microscopy revealed that fengycin BS155 caused morphological changes in the plasma membrane and cell wall of M. grisea hyphae. Using comparative proteomic and biochemical assays, fengycin BS155 was demonstrated to reduce the mitochondrial membrane potential (MMP), induce bursts of reactive oxygen species (ROS), and downregulate the expression level of ROS-scavenging enzymes. Simultaneously, fengycin BS155 caused chromatin condensation in fungal hyphal cells, which led to the upregulation of DNA repair-related protein expression and the cleavage of poly(ADP-ribose) polymerase (PARP). Altogether, our results indicate that fengycin BS155 acts by inducing membrane damage and dysfunction of organelles, disrupting MMP, oxidative stress, and chromatin condensation, resulting in M. grisea hyphal cell death. Therefore, fengycin BS155 and its parent bacterium are very promising candidates for the biological control of M. grisea and the associated rice blast and should be further investigated as such. IMPORTANCE Rice (Oryza sativa L.) is the most important crop and a primary food source for more than half of the world's population. Notably, scientists in China have developed several types of rice that can be grown in seawater, avoiding the use of precious freshwater resources and potentially creating enough food for 200 million people. The plant-affecting fungus Magnaporthe grisea is the causal agent of rice blast disease, and biological rather than chemical control of this threatening disease is highly desirable. In this work, we discovered fengycin BS155, a cyclic lipopeptide material produced by the marine bacterium Bacillus subtilis BS155, which showed strong activity against M. grisea. Our results elucidate the mechanism of fengycin BS155-mediated M. grisea growth inhibition and highlight the potential of B. subtilis BS155 as a biocontrol agent against M. grisea in rice cultivation under both fresh- and saltwater conditions.
关键词	Bacillus species lipopeptide chromatin condensation fengycin proteomic analyses reactive oxygen species production
DOI	10.1128/AEM.00445-18
关键词[WOS]	DNA-DAMAGE RESPONSE ; RHIZOPUS-STOLONIFER ; FUSARIUM-OXYSPORUM ; BIOLOGICAL-CONTROL ; OXIDATIVE STRESS ; CELL-DEATH ; APOPTOSIS ; IDENTIFICATION ; BIOCONTROL ; ANTIFUNGAL
语种	英语
资助项目	Natural Science Outstanding Youth Fund of Shandong Province[JQ201607] ; Taishan Young Scholar Program of Shandong Province[tsqn20161051] ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology[2015ASTP] ; "100-Talent Project" of the Chinese Academy of Sciences
WOS研究方向	Biotechnology & Applied Microbiology ; Microbiology
项目资助者	Natural Science Outstanding Youth Fund of Shandong Province ; Taishan Young Scholar Program of Shandong Province ; AoShan Talents Program - Qingdao National Laboratory for Marine Science and Technology ; "100-Talent Project" of the Chinese Academy of Sciences
WOS类目	Biotechnology & Applied Microbiology ; Microbiology
WOS记录号	WOS:000443291000003
出版者	AMER SOC MICROBIOLOGY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/12003
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Linlin,Sun, Chaomin. Fengycins, Cyclic Lipopeptides from Marine Bacillus subtilis Strains, Kill the Plant-Pathogenic Fungus Magnaporthe grisea by Inducing Reactive Oxygen Species Production and Chromatin Condensation[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(18):17.
APA	Zhang, Linlin,&Sun, Chaomin.(2018).Fengycins, Cyclic Lipopeptides from Marine Bacillus subtilis Strains, Kill the Plant-Pathogenic Fungus Magnaporthe grisea by Inducing Reactive Oxygen Species Production and Chromatin Condensation.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(18),17.
MLA	Zhang, Linlin,et al."Fengycins, Cyclic Lipopeptides from Marine Bacillus subtilis Strains, Kill the Plant-Pathogenic Fungus Magnaporthe grisea by Inducing Reactive Oxygen Species Production and Chromatin Condensation".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.18(2018):17.