KMS Qingdao Institute of Biomass Energy and Bioprocess Technology ,CAS
Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize | |
Wu, Zhenying1,2; Ren, Hao3; Xiong, Wangdan1,2; Roje, Sanja4; Liu, Yuchen1,2; Su, Kunlong1,2; Fu, Chunxiang1,2 | |
2018-07-20 | |
发表期刊 | JOURNAL OF EXPERIMENTAL BOTANY |
ISSN | 0022-0957 |
卷号 | 69期号:16页码:3963-3973 |
摘要 | The brown midrib2 (bm2) mutant of maize, which has a modified lignin composition, contains a mutation in the methylenetetrahydrofolate reductase (MTHFR) gene. Here, we show that a MITE transposon insertion caused downregulation of MTHFR, with an accompanying decrease in 5-methyl-tetrahydrofolate and an increase in 5, 10-methylene-tetrahydrofolate and tetrahydrofolate in the bm2 mutant. Furthermore, MTHFR mutation did not change the content of S-adenosyl methionine (SAM), the methyl group donor involved in the biosynthesis of guaiacyl and syringyl lignins, but increased the level of S-adenosyl homocysteine (SAH), the demethylation product of SAM. Moreover, competitive inhibition of the maize caffeoyl CoA O-methyltransferase (CCoAOMT) and caffeic acid O-methyltransferase (COMT) enzyme activities by SAH was found, suggesting that the SAH/SAM ratio, rather than the concentration of SAM, regulates the transmethylation reactions of lignin intermediates. Phenolic profiling revealed that caffeoyl alcohol glucose derivatives accumulated in the bm2 mutant, indicating impaired 3-O-methylation of monolignols. A remarkable increase in the unusual catechyl lignin in the mutant demonstrates that MTHFR down-regulation mainly affects guaiacyl lignin biosynthesis, consistent with the observation that CCoAOMT is more sensitive to SAH inhibition than COMT. This study uncovered a novel regulatory mechanism in lignin biosynthesis, which may offer an effective approach to utilizing lignocellulosic feedstocks in the future. |
关键词 | Lignin maize methylenetetrahydrofolate reductase methyl metabolism S-adenosyl homocysteine S-adenosyl methionine |
DOI | 10.1093/jxb/ery208 |
关键词[WOS] | ACID O-METHYLTRANSFERASE ; ONE-CARBON METABOLISM ; BROWN-MIDRIB MUTANTS ; S-ADENOSYLMETHIONINE ; HIGHER-PLANTS ; GENE ENCODES ; BIOSYNTHESIS ; MUTATION ; ARABIDOPSIS ; LIGNIFICATION |
语种 | 英语 |
资助项目 | National Key Technologies Research & Development Program-Seven Major Crops Breeding Project[2016YFD0101803] ; 100-Talent Program of the Chinese Academy of Sciences Foundation ; Natural Science Foundation of China[31470390] |
WOS研究方向 | Plant Sciences |
项目资助者 | National Key Technologies Research & Development Program-Seven Major Crops Breeding Project ; 100-Talent Program of the Chinese Academy of Sciences Foundation ; Natural Science Foundation of China |
WOS类目 | Plant Sciences |
WOS记录号 | WOS:000439796700014 |
出版者 | OXFORD UNIV PRESS |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/11897 |
专题 | 中国科学院青岛生物能源与过程研究所 |
通讯作者 | Fu, Chunxiang |
作者单位 | 1.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China 3.Nanjing Forestry Univ, Coll Light Ind & Food Engn, Nanjing 210037, Jiangsu, Peoples R China 4.Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA |
推荐引用方式 GB/T 7714 | Wu, Zhenying,Ren, Hao,Xiong, Wangdan,et al. Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(16):3963-3973. |
APA | Wu, Zhenying.,Ren, Hao.,Xiong, Wangdan.,Roje, Sanja.,Liu, Yuchen.,...&Fu, Chunxiang.(2018).Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize.JOURNAL OF EXPERIMENTAL BOTANY,69(16),3963-3973. |
MLA | Wu, Zhenying,et al."Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize".JOURNAL OF EXPERIMENTAL BOTANY 69.16(2018):3963-3973. |
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