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Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)
Liu, Xinwei1,2,3; Wang, Mengqiang1,2; Sun, Guoqiong1,2; Wang, Baojie1,2; Jiang, Keyong1,2; Shao, Jianchun1,2,3; Qi, Cancan1,2,3; Zhao, Wei4; Han, Siyin1,2,3; Liu, Mei1,2; Wang, Lei1,2
2018-10-01
Source PublicationAQUACULTURE NUTRITION
ISSN1353-5773
Volume24Issue:5Pages:1509-1520
AbstractMechanistic target of rapamycin (mTOR) is a central regulator of cell growth and metabolism including autophagy. The mTORC1 signalling pathway is a major regulator of autophagy in response to nutrient levels. The drug rapamycin induces autophagy in various cell types and species by specifically inhibiting the activity of mTOR. To identify the genes and correlative regulatory pathways involved in cell metabolism and autophagy of Litopenaeus vannamei, we analysed the transcriptome of shrimps treated with rapamycin. We found that the expression of some genes related to autophagy was significantly changed. To further explore the autophagy regulatory mechanism, the concentration and phosphorylation of related proteins were analysed by Western blot. We found that treatment with the rapamycin can depress phosphorylating of ATG1 and promote dephosphorylating of ATG13. There are some heat-shock protein genes and genes involving apoptosis, function of lysosome and feedback regulation of mTOR signalling path differently expressed. The relationship between them and autophagy induced by inhibiting mTOR is also discussed. Evidences show the complicated positive and negative feedback loops controlling autophagy crustaceans are scarce. Our study verifies and contributes to the current understanding of autophagy induced by mTOR in crustacean, providing an abundant source for the identification of novel genes.
Keywordautophagy Litopenaeus vannamei mTOR rapamycin transcriptome
DOI10.1111/anu.12688
WOS KeywordHEAT-SHOCK-RESPONSE ; CELL-GROWTH ; EMERGING ROLE ; S6 KINASE ; IN-VIVO ; PROTEIN ; MTOR ; TOR ; PHOSPHORYLATION ; APOPTOSIS
Language英语
Funding ProjectNational Natural Science Foundation of China[41406151] ; Science and Technology Major Project of Shandong Province[2015ZDZX05002] ; Chinese Academy of Sciences STS Major Deployment Project[KFZD-SW-106]
WOS Research AreaFisheries
Funding OrganizationNational Natural Science Foundation of China ; Science and Technology Major Project of Shandong Province ; Chinese Academy of Sciences STS Major Deployment Project
WOS SubjectFisheries
WOS IDWOS:000443545000014
PublisherWILEY
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/11885
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorLiu, Mei; Wang, Lei
Affiliation1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Tianjin Agr Univ, Coll Fishery Sci, Tianjin Key Lab Aquaculture, Tianjin, Peoples R China
Recommended Citation
GB/T 7714
Liu, Xinwei,Wang, Mengqiang,Sun, Guoqiong,et al. Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)[J]. AQUACULTURE NUTRITION,2018,24(5):1509-1520.
APA Liu, Xinwei.,Wang, Mengqiang.,Sun, Guoqiong.,Wang, Baojie.,Jiang, Keyong.,...&Wang, Lei.(2018).Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei).AQUACULTURE NUTRITION,24(5),1509-1520.
MLA Liu, Xinwei,et al."Mechanistic target of rapamycin inhibition with rapamycin induces autophagy and correlative regulation in white shrimp (Litopenaeus vannamei)".AQUACULTURE NUTRITION 24.5(2018):1509-1520.
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