Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai

doi:10.3389/fphys.2018.01244

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	Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai
	Jia, Yanglei 1,2,3,4; Liu, Xiao 1,2,3
	2018-09-07
发表期刊	FRONTIERS IN PHYSIOLOGY
ISSN	1664-042X
卷号	9 页码:15
摘要	Na+/K+-ATPase (NKA) belongs to the P-type ATPase family, whose members are located in the cell membrane and are distributed in diverse tissues and cells. The main function of the NKA is to regulate osmotic pressure. To better understand the role of NKA in osmoregulation, we first cloned and characterized the full-length cDNAs of NKA alpha subunit and beta subunit from Pacific abalone Haliotis discus hannai in the current study. The predicted protein sequence of the NKA a subunit, as the catalytic subunit, was well conserved. In contrast, the protein sequence of the beta subunit had low similarity with those of other species. Phylogenetic analysis revealed that both the alpha and beta subunits of the NKA protein of Pacific abalone were clustered with those of the Gastropoda. Then, the relationship between salinity changes and the NKA was investigated. Sudden salinity changes (with low-salinity seawater (LSW) or high-salinity seawater (HSW)) led to clear changes in ion concentration (Na+ and K+) in hemolymph; however, the relative stability of ion concentrations in tissue revealed that Pacific abalone has a strong osmotic pressure regulation ability when faced with these salinity changes. Meanwhile, the expression and activity of the NKA was significantly decreased (in LSW group) or increased (in HSW group) during the ion concentration re-establishing stages, which was consistent with the coordinated regulation of ion concentration in hemolymph. Moreover, a positive correlation between cyclic adenosine monophosphate (cAMP) concentrations and NKA mRNA expression (NKA activity) was observed in mantle and gill. Therefore, the sudden salinity changes may affect NKA transcription activation, translation and enzyme activity via a cAMP-mediated pathway.
关键词	salinity osmoregulation ion transportation Na+/K+-ATPase cAMP
DOI	10.3389/fphys.2018.01244
关键词[WOS]	SODIUM-PUMP REGULATION ; BETA-SUBUNIT GENES ; K+-ATPASE ; LITOPENAEUS-VANNAMEI ; CARCINUS-MAENAS ; NA,K-ATPASE ; NA+,K+-ATPASE ; GILL ; OSMOREGULATION ; PROTEIN
语种	英语
资助项目	National Natural Science Foundation of China[41676152] ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province[2017LZGC009]
WOS研究方向	Physiology
项目资助者	National Natural Science Foundation of China ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province
WOS类目	Physiology
WOS记录号	WOS:000444061000001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11805
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Liu, Xiao
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jia, Yanglei,Liu, Xiao. Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai[J]. FRONTIERS IN PHYSIOLOGY,2018,9:15.
APA	Jia, Yanglei,&Liu, Xiao.(2018).Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai.FRONTIERS IN PHYSIOLOGY,9,15.
MLA	Jia, Yanglei,et al."Expression of Na+/K+-ATPase Was Affected by Salinity Change in Pacific abalone Haliotis discus hannai".FRONTIERS IN PHYSIOLOGY 9(2018):15.