Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas

doi:10.3389/fimmu.2018.01280

QIBEBT-IR

	Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas
	Jiang, Shuai 1; Qiu, Limei 1; Wang, Lingling 2; Jia, Zhihao 1,3; Lv, Zhao 1,3; Wang, Mengqiang 1; Liu, Conghui 1,3; Xu, Jiachao 1,3; Song, Linsheng 2
	2018-06-11
发表期刊	FRONTIERS IN IMMUNOLOGY
ISSN	1664-3224
卷号	9
摘要	As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of this type from the Pacific oyster Crassostrea gigas were classified efficiently in this study via fluorescence-activated cell sorting (FACS) based on their phagocytosis of FITC-labeled latex beads. Transcriptomic and quantitative proteomic analyses revealed a series of differentially expressed genes (DEGs) and proteins present in phagocytes; of the 352 significantly high expressed proteins identified here within the phagocyte proteome, 262 corresponding genes were similarly high expressed in the transcriptome, while 140 of 205 significantly low expressed proteins within the proteome were transcriptionally low expressed. A pathway crosstalk network analysis of these significantly high expressed proteins revealed that phagocytes were highly activated in a number of antimicrobial-related biological processes, including oxidation-reduction and lysosomal proteolysis processes. A number of DEGs, including oxidase, lysosomal protease, and immune receptors, were also validated in this study using quantitative PCR, while seven lysosomal cysteine proteases, referred to as cathepsin Ls, were significantly high expressed in phagocytes. Results show that the expression level of cathepsin L protein in phagocytes [mean fluorescence intensity (MFI): 327 +/- 51] was significantly higher (p < 0.01) than that in non-phagocytic hemocytes (MFI: 83 +/- 26), while the cathepsin L protein was colocalized with the phagocytosed Vibrio splendidus in oyster hemocytes during this process. The results of this study collectively suggest that oyster phagocytes possess both potent oxidative killing and microbial disintegration capacities; these findings provide important insights into hemocyte phagocytic killing as a component of C. gigas innate immunity.
文章类型	Article
关键词	Crassostrea Gigas Transcriptome Quantitative Proteome Phagocyte Oxidative Killing Lysosome Cathepsin l
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fimmu.2018.01280
关键词[WOS]	MACROPHAGE MANNOSE RECEPTOR ; AMINO-ACID OXIDASE ; PACIFIC OYSTER ; IMMUNE-RESPONSE ; CELL-ADHESION ; MOLECULAR CHARACTERIZATION ; INVERTEBRATE IMMUNITY ; MEDIATED PHAGOCYTOSIS ; EXPRESSION ANALYSIS ; VIBRIO-SPLENDIDUS
收录类别	SCI
语种	英语
WOS研究方向	Immunology
项目资助者	Natural Science Foundation of China(31530069 ; Qingdao National Laboratory for Marine Science and Technology(2017ASTCP-OS13) ; 863 Program(2014AA103501) ; for Modern Agro-industry Technology Research System(CARS-49) ; Dalian High Level Talent Innovation Support Program(2015R020) ; Research Foundation for Distinguished Professor in Liaoning ; Talented Scholars in Dalian Ocean University ; 41406170)
WOS类目	Immunology
WOS记录号	WOS:000434796400001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11438
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Wang, Lingling
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Dalian Ocean Univ, Liaoning Key Lab Marine Anim Immunol & Dis Contro, Dalian, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Shuai,Qiu, Limei,Wang, Lingling,et al. Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas[J]. FRONTIERS IN IMMUNOLOGY,2018,9.
APA	Jiang, Shuai.,Qiu, Limei.,Wang, Lingling.,Jia, Zhihao.,Lv, Zhao.,...&Song, Linsheng.(2018).Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.FRONTIERS IN IMMUNOLOGY,9.
MLA	Jiang, Shuai,et al."Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas".FRONTIERS IN IMMUNOLOGY 9(2018).