Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor

doi:10.1016/j.bios.2018.03.058

QIBEBT-IR

	Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor
	Hu, Liang 1,2,3; Ge, Anle 1,2,4,5; Wang, Xixian 1,2,4,5; Wang, Shanshan 1,2; Yue, Xinpei 6; Wang, Jie 1,2; Feng, Xiaojun 1,2; Du, Wei 1,2; Liu, Bi-Feng 1,2
	2018-07-01
发表期刊	BIOSENSORS & BIOELECTRONICS
ISSN	0956-5663
卷号	110 页码:233-238
摘要	Immune response to environmental pathogen invasion is a complex process to prevent host from further damage. For quantitatively understanding immune responses and revealing the pathogenic environmental information, real-time monitoring of such a whole dynamic process with single-animal resolution in well-defined environments is highly desired. In this work, an integrated microfluidic device coupled with worm-based biosensor was proposed for in vivo studies of dynamic immune responses and antibiotics interference in infected C. elegans. Individual worms housed in chambers were exposed to the various pathogens and discontinuously manipulated for imaging with limited influence on physiological activities. The expression of immune responses gene (irg-1) was time-lapse measured in intact worms during pathogen infection. Results demonstrated that irg-1 gene could be induced in the presence of P. aeruginosa strain PA14 in a dose-dependent manner, and the survival of infected worm could be rescued under gentamicin or erythromycin treatments. We expect it to be a versatile platform to facilitate future studies on pathogenesis researches and rapid drug screen using C. elegans disease model.
文章类型	Article
关键词	Microfluidic Chip Worm-based Biosensor Immune Responses In Vivo Analysis Drug Evaluation
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
DOI	10.1016/j.bios.2018.03.058
关键词[WOS]	ANIMAL INFECTION MODEL ; C.-ELEGANS MODEL ; CAENORHABDITIS-ELEGANS ; IN-VIVO ; PSEUDOMONAS-AERUGINOSA ; INNATE IMMUNITY ; NEURONAL-ACTIVITY ; CHIP ; ERYTHROMYCIN ; DISCOVERY
收录类别	SCI
语种	英语
WOS研究方向	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
项目资助者	National Key R&D Program of China(2016YFF0100801) ; National Natural Science Foundation of China(21475049 ; Fundamental Research Funds for the Central Universities(2016YXZD061) ; 31471257 ; 21275060)
WOS类目	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
WOS记录号	WOS:000432613300031
出版者	ELSEVIER ADVANCED TECHNOLOGY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11430
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Du, Wei; Liu, Bi-Feng
作者单位	1.Huazhong Univ Sci & Technol, Key Lab Biomed Photon, MOE, Wuhan Natl Lab Optoelect Hubei Bioinformat, Wuhan 430074, Hubei, Peoples R China 2.Huazhong Univ Sci & Technol, Mol Imaging Key Lab, Syst Biol Theme, Dept Biomed Engn,Coll Life Sci & Technol, Wuhan 430074, Hubei, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Phys & Math, Brain Res Ctr, Wuhan 430071, Hubei, Peoples R China 4.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China 6.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Hu, Liang,Ge, Anle,Wang, Xixian,et al. Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor[J]. BIOSENSORS & BIOELECTRONICS,2018,110:233-238.
APA	Hu, Liang.,Ge, Anle.,Wang, Xixian.,Wang, Shanshan.,Yue, Xinpei.,...&Liu, Bi-Feng.(2018).Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor.BIOSENSORS & BIOELECTRONICS,110,233-238.
MLA	Hu, Liang,et al."Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor".BIOSENSORS & BIOELECTRONICS 110(2018):233-238.