MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions

doi:10.1016/j.molp.2018.03.013

QIBEBT-IR

	MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions
	Sun, Qing 1; Liu, Xiaogang 1; Yang, Juan 1; Liu, Wenwen ; Du, Qingguo 1; Wang, Hongqiu 1; Fu, Chunxiang 2,3; Li, Wen-Xue 1
	2018-06-04
发表期刊	MOLECULAR PLANT
ISSN	1674-2052
卷号	11 期号:6 页码:806-814
摘要	Lodging under nitrogen (N)-luxury conditions substantially reduces crop yield and seed quality. However, the molecular mechanisms of plant lodging resistance remain largely unclear, especially in maize. We report here that the expression of ZmmiR528, a monocot-specific microRNA, is induced by N luxury but reduced by N deficiency. We show by the thioacidolysis and acetyl bromide analysis that N luxury significantly reduces the generation of H, G, and S monomers of the lignin as well as its total content in maize shoots. We further demonstrate that ZmLACCASE3 (ZmLAC3) and ZmLACCASE5 (ZmLAC5), which encode the copper-containing laccases, are the targets of ZmmiR528. In situ hybridization showed that ZmmiR528 is mainly expressed in maize vascular tissues. Knockdown of ZmmiR528 or overexpression of ZmLAC3 significantly increased the lignin content and rind penetrometer resistance of maize stems. In contrast, transgenic maize plants overexpressing ZmmiR528 had reduced lignin content and rind penetrometer resistance and were prone to lodging under N-luxury conditions. RNA-sequencing analysis revealed that ZmPAL7 and ZmPAL8 are upregulated in transgenic maize lines downregulating ZmmiR528. Under N-luxury conditions, the expression levels of ZmPALs were much higher in ZmmiR528-knockdown lines than in the wild type and transgenic maize lines overexpressing ZmmiR528. Taken together, these results indicate that, by regulating the expression ofZmLAC3 and ZmLAC5, ZmmiR528 affects maize lodging resistance under N-luxury conditions.
文章类型	Article
关键词	Maize Nitrogen Zmmir528 Zmlacs Lodging Resistance
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.molp.2018.03.013
关键词[WOS]	ARABIDOPSIS-THALIANA ROOTS ; CELL-WALL LIGNIFICATION ; TRANSCRIPTION FACTORS ; NITRATE RESPONSE ; GENE-EXPRESSION ; LACCASE ; PLANTS ; SYSTEMS ; FERTILIZATION ; MANAGEMENT
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences
项目资助者	National Key Research and Development Program of China(2016YFD0100701) ; Ministry of Agriculture of China for Transgenic Research(2018ZX0800988B) ; Agricultural Science and Technology Innovation Program of CAAS
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences
WOS记录号	WOS:000434429000005
出版者	CELL PRESS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11410
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Li, Wen-Xue
作者单位	1.Chinese Acad Agr Sci, Inst Crop Sci, Natl Engn Lab Crop Mol Breeding, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Biofuels, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Sun, Qing,Liu, Xiaogang,Yang, Juan,et al. MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions[J]. MOLECULAR PLANT,2018,11(6):806-814.
APA	Sun, Qing.,Liu, Xiaogang.,Yang, Juan.,Liu, Wenwen.,Du, Qingguo.,...&Li, Wen-Xue.(2018).MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions.MOLECULAR PLANT,11(6),806-814.
MLA	Sun, Qing,et al."MicroRNA528 Affects Lodging Resistance of Maize by Regulating Lignin Biosynthesis under Nitrogen-Luxury Conditions".MOLECULAR PLANT 11.6(2018):806-814.