Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification

doi:10.1186/s13568-018-0622-3

QIBEBT-IR

	Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification
	Wang, Yanli 1; Chen, Quan 2; Xian, Mo 2; Nian, Rui 2; Xu, Fei 1
	2018-06-01
发表期刊	AMB EXPRESS
ISSN	2191-0855
卷号	8
摘要	In recent studies, electronegative multimodal chromatography with Eshmuno HCX was demonstrated to be a highly promising recovery step for direct immunoglobulin G (IgG) capture from undiluted cell culture fluid. In this study, the binding properties of HCX to IgG at different pH/salt combinations were systematically studied, and its purification performance was significantly enhanced by lowering the washing pH and conductivity after high capacity binding of IgG under its optimal conditions. A single polishing step gave an end-product with non-histone host cell protein (nh-HCP) below 1 ppm, DNA less than 1 ppb, which aggregates less than 0.5% and an overall IgG recovery of 86.2%. The whole non-affinity chromatography based two-column-step process supports direct feed loading without buffer adjustment, thus extraordinarily boosting the overall productivity and cost-savings.
文章类型	Article
关键词	Monoclonal Antibody Electronegative Multimodal Non-affinity Purification Cost-savings
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1186/s13568-018-0622-3
关键词[WOS]	CATION-EXCHANGE CHROMATOGRAPHY ; HYDROPHOBIC INTERACTION CHROMATOGRAPHY ; PROTEIN-A CHROMATOGRAPHY ; CELL-CULTURE FLUID ; MONOCLONAL-ANTIBODIES ; VOID-EXCLUSION ; CHROMATIN ; PERFORMANCE ; PH ; CLEARANCE
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	National Natural Science Foundation of China(21676286)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000433978500004
出版者	BIOMED CENTRAL LTD
引用统计	被引频次：2[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11392
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Nian, Rui; Xu, Fei
作者单位	1.Jilin Univ, Coll Life Sci, Changchun, Jilin, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yanli,Chen, Quan,Xian, Mo,et al. Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification[J]. AMB EXPRESS,2018,8.
APA	Wang, Yanli,Chen, Quan,Xian, Mo,Nian, Rui,&Xu, Fei.(2018).Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification.AMB EXPRESS,8.
MLA	Wang, Yanli,et al."Application of enhanced electronegative multimodal chromatography as the primary capture step for immunoglobulin G purification".AMB EXPRESS 8(2018).