Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?

doi:10.3389/fpls.2018.01545

QIBEBT-IR

	Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?
	Lin, Haiying 1; Sun, Tao 1; Zhou, Yi 2; Gu, Ruiting 2; Zhang, Xiaomei 2; Yang, Wei 1
	2018-10-24
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	9 页码:12
摘要	Healthy seagrasses are considered a prime indicator of estuarine and coastal ecosystem function; however, as the only group of flowering plants recolonizing the sea, seagrasses are frequently exposed to anthropogenic heavy metal pollutants, which are associated with high levels of molecular damage. To determine whether biologically relevant concentrations of heavy metals cause systematic alterations in RNA expression patterns, we performed a gene expression study using transcriptome analyses (RNA-seq). We exposed the typical intertidal seagrass Zostera japonica to 0 and 50 mu M of copper (Cu), lead (Pb), and cadmium (Cd) under laboratory conditions. A total of 18,266 differentially expressed genes (DEGs) were identified, of which 2001 co-expressed genes directly related by Cu, Pb, and Cd stress. We also examined the effects of short-term heavy metal Cu, Pb, and Cd pulses on the accumulation of metals in Z. japonica and showed metal concentrations were higher in the shoots than in roots. Twelve differentially expressed genes were further analyzed for expression differences using real-time quantitative polymerase chain reaction (RT-qPCR). Our data suggest that as coastal seawater pollution worsens, the sensitive genes identified in this study may be useful biomarkers of sublethal effects and provide fundamental information for Z. japonica resistant gene engineering.
关键词	genes transcriptomic analysis heavy metals seagrass Zostera japonica
DOI	10.3389/fpls.2018.01545
关键词[WOS]	EXPRESSED SEQUENCE TAGS ; POSIDONIA-OCEANICA ; RNA-SEQ ; TRANSCRIPTOME ANALYSIS ; TEMPERATURE-STRESS ; LIGHT CONDITIONS ; MARINA ; TOLERANCE ; ECOSYSTEMS ; EXPOSURE
语种	英语
资助项目	National Key Research and Development Program of China[2018YFC1406404] ; National Marine Public Welfare Research Project[201305043] ; National Science & Technology Basic Work Program[2015FY110600] ; NSFC-Shandong Joint Fund for Marine Science Research Centers[U1606404] ; key Research and Development Project of Shandong Province[2017GHY15111] ; Key Research Project of Frontier Science of Chinese Academy of Sciences[QYZDB-SSWDQC041-1] ; National Natural Science Foundation of China[41176140]
WOS研究方向	Plant Sciences
项目资助者	National Key Research and Development Program of China ; National Marine Public Welfare Research Project ; National Science & Technology Basic Work Program ; NSFC-Shandong Joint Fund for Marine Science Research Centers ; key Research and Development Project of Shandong Province ; Key Research Project of Frontier Science of Chinese Academy of Sciences ; National Natural Science Foundation of China
WOS类目	Plant Sciences
WOS记录号	WOS:000448111500001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11345
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Tao; Zhou, Yi
作者单位	1.Beijing Normal Univ, State Key Lab Water Environm Simulat, Sch Environm, Beijing, Peoples R China 2.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Lin, Haiying,Sun, Tao,Zhou, Yi,et al. Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?[J]. FRONTIERS IN PLANT SCIENCE,2018,9:12.
APA	Lin, Haiying,Sun, Tao,Zhou, Yi,Gu, Ruiting,Zhang, Xiaomei,&Yang, Wei.(2018).Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?.FRONTIERS IN PLANT SCIENCE,9,12.
MLA	Lin, Haiying,et al."Which Genes in a Typical Intertidal Seagrass (Zostera japonica) Indicate Copper-, Lead-, and Cadmium Pollution?".FRONTIERS IN PLANT SCIENCE 9(2018):12.