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Title:
Bio-isopreneproduction using exogenous MVA pathway and isoprenesynthase in Escherichia coli
Author: Jianming Yang ; Guang Zhao ; Yuanzhang Sun ; Xinglin Jiang ; Mo Xian.
Source: Bioresource Technology
Issued Date: 2012-01
Volume: 104, Issue:1, Pages:642–647
Subject: 生物材料::生物基化学品
Abstract: In this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiaeMVApathway and isoprenesynthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVApathway and ispSPa gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVApathway in E. coli has been provided.
Language: 英语
Content Type: 期刊论文
URI: http://ir.qibebt.ac.cn/handle/337004/1116
Appears in Collections:材料生物技术研究中心_期刊论文

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Recommended Citation:
Jianming Yang,Guang Zhao,Yuanzhang Sun,et al. Bio-isopreneproduction using exogenous MVA pathway and isoprenesynthase in Escherichia coli[J]. Bioresource Technology,2012,104(1):642–647.
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文件名: Bio-isopreneproduction using exogenousMVApathway and isoprenesynthase in Escherichia coli.pdf
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