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Bio-isopreneproduction using exogenous MVA pathway and isoprenesynthase in Escherichia coli
Jianming Yang; Guang Zhao; Yuanzhang Sun; Xinglin Jiang; Mo Xian.
2012-01
Source PublicationBioresource Technology
Volume104Issue:1Pages:642–647
AbstractIn this paper, an original strategy is employed to biosynthesize the isoprene by heterologously co-expressing the Saccharomyces cerevisiaeMVApathway and isoprenesynthase (IspS) from Populus alba in the Escherichia coli BL21 (DE3) strain, which was screened from three different IspS enzymes. The finally genetic strain YJM13 harboring the MVApathway and ispSPa gene could accumulate isoprene up to 2.48 mg/l and 532 mg/l under the flask and fed-batch fermentation conditions, respectively, which is about three times and five times to the control strain. The result proves to be higher than that in the report documents. In this way, a potential production system for isoprene from renewable sources via the MVApathway in E. coli has been provided.
Subject Area生物基化学品
Language英语
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/1116
Collection材料生物技术研究中心
Recommended Citation
GB/T 7714
Jianming Yang,Guang Zhao,Yuanzhang Sun,et al. Bio-isopreneproduction using exogenous MVA pathway and isoprenesynthase in Escherichia coli[J]. Bioresource Technology,2012,104(1):642–647.
APA Jianming Yang,Guang Zhao,Yuanzhang Sun,Xinglin Jiang,&Mo Xian..(2012).Bio-isopreneproduction using exogenous MVA pathway and isoprenesynthase in Escherichia coli.Bioresource Technology,104(1),642–647.
MLA Jianming Yang,et al."Bio-isopreneproduction using exogenous MVA pathway and isoprenesynthase in Escherichia coli".Bioresource Technology 104.1(2012):642–647.
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