| Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography | |
Feng, Dexin1; Li, Liangzhi1; Yang, Fang1; Tan, Weiqiang1; Zhao, Guoming1; Zou, Huibin1; Xian, Mo1 ; Zhang, Yingwei2
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| 2011-07-01 | |
| 发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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| 卷号 | 91期号:2页码:399-405 |
| 摘要 | Pretreatment of cellulose with ionic liquids (ILs) can improve the efficiency of the hydrolysis by increasing the surface area of the substrates accessible to solvents and cellulases. However, the IL methods are facing challenges to separate the hydrolyzed sugar products as well as the renewable ILs from the complex hydrolysis mixtures. In this study, an alumina column chromatography (ACC) method was developed for the separation of hydrophilic IL N-methyl-N-methylimidazolium dimethyl phosphate ([Mmim][DMP]) and glucose, which was the main ingredient of the monosaccharide hydrolyzate. The processing parameters involved in ACC separation were investigated in detail. Our results showed that the recovery yields of [Mmim][DMP] and glucose can reach up to 93.38% and 90.14%, respectively, under the optimized parameters: the sampling ratio of 1:20 between the applied sample volume and the bed volume of the column; a gradient elution using methanol (100%, 150 ml) and then water (170 ml) as eluents with 1 ml/min flow rate. The recovered [Mmim][DMP] showed qualified property and was effective in a new hydrolysis reaction. In addition, scale-up ACC separations were successfully done with satisfied separation performance. The results indicated that the ACC is one of the available methods for the separation of ILs and monosaccharides from the hydrolysis mixtures.; Pretreatment of cellulose with ionic liquids (ILs) can improve the efficiency of the hydrolysis by increasing the surface area of the substrates accessible to solvents and cellulases. However, the IL methods are facing challenges to separate the hydrolyzed sugar products as well as the renewable ILs from the complex hydrolysis mixtures. In this study, an alumina column chromatography (ACC) method was developed for the separation of hydrophilic IL N-methyl-N-methylimidazolium dimethyl phosphate ([Mmim][DMP]) and glucose, which was the main ingredient of the monosaccharide hydrolyzate. The processing parameters involved in ACC separation were investigated in detail. Our results showed that the recovery yields of [Mmim][DMP] and glucose can reach up to 93.38% and 90.14%, respectively, under the optimized parameters: the sampling ratio of 1:20 between the applied sample volume and the bed volume of the column; a gradient elution using methanol (100%, 150 ml) and then water (170 ml) as eluents with 1 ml/min flow rate. The recovered [Mmim][DMP] showed qualified property and was effective in a new hydrolysis reaction. In addition, scale-up ACC separations were successfully done with satisfied separation performance. The results indicated that the ACC is one of the available methods for the separation of ILs and monosaccharides from the hydrolysis mixtures. |
| 文章类型 | Article |
| 关键词 | Ionic Liquids Hydrophilic Recycle Alumina Column Chromatography Monosaccharide |
| 学科领域 | 生物基化学品 |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| DOI | 10.1007/s00253-011-3263-x |
| 关键词[WOS] | IN-SITU SACCHARIFICATION ; AQUEOUS BIPHASIC SYSTEMS ; GREEN SOLVENTS ; WATER ; HYDROCARBONS ; PRETREATMENT ; ENHANCEMENT ; ADSORPTION ; EXTRACTION ; INTERFACE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| WOS类目 | Biotechnology & Applied Microbiology |
| WOS记录号 | WOS:000292203200016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.qibebt.ac.cn/handle/337004/1111 |
| 专题 | 生物基材料组群 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuel, Qingdao 266101, Peoples R China 2.Risun Coal Chem Grp Ltd, Xingtai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Dexin,Li, Liangzhi,Yang, Fang,et al. Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2011,91(2):399-405. |
| APA | Feng, Dexin.,Li, Liangzhi.,Yang, Fang.,Tan, Weiqiang.,Zhao, Guoming.,...&Zhang, Yingwei.(2011).Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,91(2),399-405. |
| MLA | Feng, Dexin,et al."Separation of ionic liquid [Mmim][DMP] and glucose from enzymatic hydrolysis mixture of cellulose using alumina column chromatography".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 91.2(2011):399-405. |
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