Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis

doi:10.6023/cjoc201805055

QIBEBT-IR

	Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis
	Jiang Yuanyuan 1,2; Li Shengying 1,2
	2018-09-25
发表期刊	CHINESE JOURNAL OF ORGANIC CHEMISTRY
ISSN	0253-2786
卷号	38 期号:9 页码:2307-2323
摘要	Cytochrome P450 enzymes are widely distributed in nature, which mainly participate in xenobiotics metabolism and natural product biosynthesis. These enzymes are able to recognize various substrates to produce many useful oxidative products through diverse reaction types. P450 enzymes can catalyze selective oxidation of C-H bonds in their substrates under mild conditions. Therefore, a lot of P450 enzymes have been applied in the production of fine chemicals, drugs and chemical intermediates for quite a long time. With the development of protein engineering, redox partner engineering, substrate engineering, metabolic engineering and synthetic biology, it has become possible to obtain the P450 biocatalysts with the desired properties such as high activity, the substrate specificity of interest, and great selectivity to meet the industrial requirements, through rational design and direct evolution of P450 enzymes. Thus, the application scope of P450 enzymes in biosynthesis and organic synthesis has been expanded greatly. The types of reactions that can be catalyzed by P450 enzymes, and the strategies to broaden the reaction scope and to enhance the catalytic efficiency and selectivity are summarized. Finally, the challenges and prospects in the application of cytochrome P450 enzymes in biosynthesis and organic synthesis are discussed.
关键词	cytochrome P450 enzyme biosynthesis organic synthesis protein engineering substrate engineering metabolic engineering synthetic biology redox partner
DOI	10.6023/cjoc201805055
关键词[WOS]	CELL BIOTRANSFORMATION SYSTEM ; DIRECTED EVOLUTION ; NATURAL-PRODUCTS ; P450 ENZYMES ; SATURATION MUTAGENESIS ; ALKALOID BIOSYNTHESIS ; MOLECULAR-CLONING ; LIVER-MICROSOMES ; TERPENE CYCLASE ; HYDROXYLATION
语种	英语
资助项目	Natural Science Foundation of Shandong Province[ZR2017ZB0207] ; National Natural Science Foundation of China[81741115] ; National Natural Science Foundation of China[21472204]
WOS研究方向	Chemistry
项目资助者	Natural Science Foundation of Shandong Province ; National Natural Science Foundation of China
WOS类目	Chemistry, Organic
WOS记录号	WOS:000450205800009
出版者	SCIENCE PRESS
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11053
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Li Shengying
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Jiang Yuanyuan,Li Shengying. Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis[J]. CHINESE JOURNAL OF ORGANIC CHEMISTRY,2018,38(9):2307-2323.
APA	Jiang Yuanyuan,&Li Shengying.(2018).Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis.CHINESE JOURNAL OF ORGANIC CHEMISTRY,38(9),2307-2323.
MLA	Jiang Yuanyuan,et al."Catalytic Function and Application of Cytochrome P450 Enzymes in Biosynthesis and Organic Synthesis".CHINESE JOURNAL OF ORGANIC CHEMISTRY 38.9(2018):2307-2323.