QIBEBT-IR
Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide
Liang, Bo1,2,3,4; Wang, Ge1,2,5; Yan, Lu1,2; Ren, Han6; Feng, Ruirui3,4; Xiong, Zhong5; Liu, Aihua1,2,5,6
2019-01-15
Source PublicationSENSORS AND ACTUATORS B-CHEMICAL
ISSN0925-4005
Volume279Pages:483-489
AbstractThe inhibition of acetylcholinesterase (AChE) activity based methods have been widely used for rapid detection of organophosphate (OP) pesticide residues. However, the sensitivity of the enzyme toward pesticides is not desirable and complex purification steps increase production cost. In this study, we optimized and synthesized the cDNA of carp AChE based on the preferred codon using Saccharomyces cerevisiae, which was ligated into expression vector pYD1. The AChE was successfully presented on yeast surface through a-agglutinin mediated display system. In shake-flask after induction with galactose in YPD medium for 24 h, this strain expressed AChE activity at a high level of 0.15 U/OD600/ml. The correct location of AChE was confirmed by immunofluorescence analysis. The whole cell catalyst was applied to visible spectrophotometry detection for OP pesticides based on inhibition rate. The inhibition rate was linear with paraoxon concentration in the range of 0.5 ng/ml-10 mu g/ml and parathion concentration in the range of 5 ng/ml-10 mu g/ml. The low detection limit was 0.136 ng/ml paraoxon and 3.72 ng/ml parathion (S/N = 3). The proposed method is applicable to analyzing OP pesticides in real samples. Therefore, the recombinant strain developed could be an efficient and affordable biomaterial for rapid and accurate detection of OP pesticide residues.
KeywordCarp Acetylcholinesterase (AChE) Yeast Cell surface display Organophosphate pesticide detection
DOI10.1016/j.snb.2018.09.119
WOS KeywordMICROBIAL BIOSENSOR ; SENSITIVE ACETYLCHOLINESTERASE ; NIPPOSTRONGYLUS-BRASILIENSIS ; GLUTAMATE-DEHYDROGENASE ; GLUCOSE-DEHYDROGENASE ; XYLOSE DEHYDROGENASE ; PICHIA-PASTORIS ; NERVE AGENTS ; HYDROLASE ; EXPRESSION
Language英语
Funding ProjectNational Natural Science Foundation of China[81673172] ; National Natural Science Foundation of China[21475144] ; National Natural Science Foundation of China[91227116] ; National Natural Science Foundation of China[21275152] ; Major Program of Shandong Province Natural Science Foundation[ZR2018ZC0125]
WOS Research AreaChemistry ; Electrochemistry ; Instruments & Instrumentation
Funding OrganizationNational Natural Science Foundation of China ; Major Program of Shandong Province Natural Science Foundation
WOS SubjectChemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS IDWOS:000449373800060
PublisherELSEVIER SCIENCE SA
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/11011
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorLiu, Aihua
Affiliation1.Qingdao Univ, Inst Biosensing, 308 Ningxia Rd, Qingdao 266071, Peoples R China
2.Qingdao Univ, Coll Life Sci, 308 Ningxia Rd, Qingdao 266071, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, 189 Songling Rd, Qingdao 266101, Peoples R China
4.Chinese Acad Sci, Key Lab Bioenergy, 189 Songling Rd, Qingdao 266101, Peoples R China
5.Qingdao Univ, Coll Chem & Chem Engn, Qingdao 266071, Peoples R China
6.Qingdao Univ, Med Coll, Sch Pharm, Dept Drug Metab & Anal, Qingdao 266021, Peoples R China
Recommended Citation
GB/T 7714
Liang, Bo,Wang, Ge,Yan, Lu,et al. Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide[J]. SENSORS AND ACTUATORS B-CHEMICAL,2019,279:483-489.
APA Liang, Bo.,Wang, Ge.,Yan, Lu.,Ren, Han.,Feng, Ruirui.,...&Liu, Aihua.(2019).Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide.SENSORS AND ACTUATORS B-CHEMICAL,279,483-489.
MLA Liang, Bo,et al."Functional cell surface displaying of acetylcholinesterase for spectrophotometric sensing organophosphate pesticide".SENSORS AND ACTUATORS B-CHEMICAL 279(2019):483-489.
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