QIBEBT-IR
Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass
Bai, Zetao1,2,3; Qi, Tianxiong1,2,3; Liu, Yuchen1,2,3; Wu, Zhenying1,2,3; Ma, Lichao1,2,3; Liu, Wenwen1,2,3; Cao, Yingping1,2,3; Bao, Yan1,2,3; Fu, Chunxiang1,2,3
2018-12-01
Source PublicationPLANT BIOTECHNOLOGY JOURNAL
ISSN1467-7644
Volume16Issue:12Pages:2016-2026
AbstractMethionine (Met) synthesized from aspartate is a fundamental amino acid needed to produce S-adenosylmethionine (SAM) that is an important cofactor for the methylation of monolignols. As a competitive inhibitor of SAM-dependent methylation, the effect of S-adenosylhomocysteine (SAH) on lignin biosynthesis, however, is still largely unknown in plants. Expression levels of Cystathionine gamma-synthase (PvCGS) and S-adenosylhomocysteine hydrolase 1 (PvSAHH1) were down-regulated by RNAi technology, respectively, in switchgrass, a dual-purpose forage and biofuel crop. The transgenic switchgrass lines were subjected to studying the impact of SAH on lignin biosynthesis. Our results showed that down-regulation of PvCGS in switchgrass altered the accumulation of aspartate-derived and aromatic amino acids, reduced the content of SAH, enhanced lignin biosynthesis and stunted plant growth. In contrast, down-regulation of PvSAHH1 raised SAH levels in switchgrass, impaired the biosynthesis of both guaiacyl and syringyl lignins and therefore significantly increased saccharification efficiency of cell walls. This work indicates that SAH plays a crucial role in monolignol methylation in switchgrass. Genetic regulation of either PvCGS or PvSAHH1 expression in switchgrass can change intracellular SAH contents and SAM to SAH ratios and therefore affect lignin biosynthesis. Thus, our study suggests that genes involved in Met metabolism are of interest as new valuable targets for cell wall bioengineering in future.
Keywordcystathionine gamma-synthase lignin biosynthesis methionine metabolism Panicum virgatum L. (switchgrass) S-adenosylhomocysteine S-adenosylhomocysteine hydrolase S-adenosylmethionine
DOI10.1111/pbi.12935
WOS KeywordCYSTATHIONINE-GAMMA-SYNTHASE ; ADENOSYL-L-METHIONINE ; PANICUM VIRGATUM L. ; METHYLMETHIONINE CYCLE ; FUNCTIONAL-ANALYSIS ; SOLUBLE METHIONINE ; BIOFUEL PRODUCTION ; POTATO PLANTS ; RNA-SEQ ; ARABIDOPSIS
Language英语
Funding Project'100-Talent Program of the Chinese Academy of Sciences' foundation ; National Natural Science Foundation of China[31470390] ; National Natural Science Foundation of China[31700258] ; National Key Technologies Research & Development Program-Seven major crops breeding project[2016YFD0101803] ; Science and Technology Major Project of Shandong[2016ZDJS10A04]
WOS Research AreaBiotechnology & Applied Microbiology ; Plant Sciences
Funding Organization'100-Talent Program of the Chinese Academy of Sciences' foundation ; National Natural Science Foundation of China ; National Key Technologies Research & Development Program-Seven major crops breeding project ; Science and Technology Major Project of Shandong
WOS SubjectBiotechnology & Applied Microbiology ; Plant Sciences
WOS IDWOS:000449663300005
PublisherWILEY
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/11008
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorFu, Chunxiang
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Shandong, Peoples R China
3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao, Shandong, Peoples R China
Recommended Citation
GB/T 7714
Bai, Zetao,Qi, Tianxiong,Liu, Yuchen,et al. Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass[J]. PLANT BIOTECHNOLOGY JOURNAL,2018,16(12):2016-2026.
APA Bai, Zetao.,Qi, Tianxiong.,Liu, Yuchen.,Wu, Zhenying.,Ma, Lichao.,...&Fu, Chunxiang.(2018).Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass.PLANT BIOTECHNOLOGY JOURNAL,16(12),2016-2026.
MLA Bai, Zetao,et al."Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass".PLANT BIOTECHNOLOGY JOURNAL 16.12(2018):2016-2026.
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