Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass

doi:10.1111/pbi.12935

QIBEBT-IR

	Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass
	Bai, Zetao 1,2,3; Qi, Tianxiong 1,2,3; Liu, Yuchen 1,2,3; Wu, Zhenying 1,2,3; Ma, Lichao 1,2,3; Liu, Wenwen 1,2,3; Cao, Yingping 1,2,3; Bao, Yan 1,2,3; Fu, Chunxiang1,2,3
	2018-12-01
发表期刊	PLANT BIOTECHNOLOGY JOURNAL
ISSN	1467-7644
卷号	16 期号:12 页码:2016-2026
摘要	Methionine (Met) synthesized from aspartate is a fundamental amino acid needed to produce S-adenosylmethionine (SAM) that is an important cofactor for the methylation of monolignols. As a competitive inhibitor of SAM-dependent methylation, the effect of S-adenosylhomocysteine (SAH) on lignin biosynthesis, however, is still largely unknown in plants. Expression levels of Cystathionine gamma-synthase (PvCGS) and S-adenosylhomocysteine hydrolase 1 (PvSAHH1) were down-regulated by RNAi technology, respectively, in switchgrass, a dual-purpose forage and biofuel crop. The transgenic switchgrass lines were subjected to studying the impact of SAH on lignin biosynthesis. Our results showed that down-regulation of PvCGS in switchgrass altered the accumulation of aspartate-derived and aromatic amino acids, reduced the content of SAH, enhanced lignin biosynthesis and stunted plant growth. In contrast, down-regulation of PvSAHH1 raised SAH levels in switchgrass, impaired the biosynthesis of both guaiacyl and syringyl lignins and therefore significantly increased saccharification efficiency of cell walls. This work indicates that SAH plays a crucial role in monolignol methylation in switchgrass. Genetic regulation of either PvCGS or PvSAHH1 expression in switchgrass can change intracellular SAH contents and SAM to SAH ratios and therefore affect lignin biosynthesis. Thus, our study suggests that genes involved in Met metabolism are of interest as new valuable targets for cell wall bioengineering in future.
关键词	cystathionine gamma-synthase lignin biosynthesis methionine metabolism Panicum virgatum L. (switchgrass) S-adenosylhomocysteine S-adenosylhomocysteine hydrolase S-adenosylmethionine
DOI	10.1111/pbi.12935
关键词[WOS]	CYSTATHIONINE-GAMMA-SYNTHASE ; ADENOSYL-L-METHIONINE ; PANICUM VIRGATUM L. ; METHYLMETHIONINE CYCLE ; FUNCTIONAL-ANALYSIS ; SOLUBLE METHIONINE ; BIOFUEL PRODUCTION ; POTATO PLANTS ; RNA-SEQ ; ARABIDOPSIS
语种	英语
资助项目	'100-Talent Program of the Chinese Academy of Sciences' foundation ; National Natural Science Foundation of China[31470390] ; National Natural Science Foundation of China[31700258] ; National Key Technologies Research & Development Program-Seven major crops breeding project[2016YFD0101803] ; Science and Technology Major Project of Shandong[2016ZDJS10A04]
WOS研究方向	Biotechnology & Applied Microbiology ; Plant Sciences
项目资助者	'100-Talent Program of the Chinese Academy of Sciences' foundation ; National Natural Science Foundation of China ; National Key Technologies Research & Development Program-Seven major crops breeding project ; Science and Technology Major Project of Shandong
WOS类目	Biotechnology & Applied Microbiology ; Plant Sciences
WOS记录号	WOS:000449663300005
出版者	WILEY
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/11008
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Fu, Chunxiang
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Energy Genet, Qingdao, Shandong, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Key Lab Biofuels, Qingdao, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Bai, Zetao,Qi, Tianxiong,Liu, Yuchen,et al. Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass[J]. PLANT BIOTECHNOLOGY JOURNAL,2018,16(12):2016-2026.
APA	Bai, Zetao.,Qi, Tianxiong.,Liu, Yuchen.,Wu, Zhenying.,Ma, Lichao.,...&Fu, Chunxiang.(2018).Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass.PLANT BIOTECHNOLOGY JOURNAL,16(12),2016-2026.
MLA	Bai, Zetao,et al."Alteration of S-adenosylhomocysteine levels affects lignin biosynthesis in switchgrass".PLANT BIOTECHNOLOGY JOURNAL 16.12(2018):2016-2026.