QIBEBT-IR
Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli
Liu, Changqing1,2; Zhang, Kai1,2; Cao, Wenyan1,2; Zhang, Ge1,2,3; Chen, Guoqiang1,2,3; Yang, Haiyan1,2; Wang, Qian3; Liu, Haobao3; Xian, Mo1,2; Zhang, Haibo1,2
2018-11-08
Source PublicationBIOTECHNOLOGY FOR BIOFUELS
ISSN1754-6834
Volume11Pages:15
AbstractBackground2-Phenylethanol (2-PE) is a higher aromatic alcohol that is widely used in the perfumery, cosmetics, and food industries and is also a potentially valuable next-generation biofuel. In our previous study, a new strain Enterobacter sp. CGMCC 5087 was isolated to produce 2-PE from glucose through the phenylpyruvate pathway.ResultsIn this study, candidate genes for 2-PE biosynthesis were identified from Enterobacter sp. CGMCC 5087 by draft whole-genome sequence, metabolic engineering, and shake flask fermentation. Subsequently, the identified genes encoding the 2-keto acid decarboxylase (Kdc) and alcohol dehydrogenase (Adh) enzymes from Enterobacter sp. CGMCC 5087 were introduced into E. coli BL21(DE3) to construct a high-efficiency microbial cell factory for 2-PE production using the prokaryotic phenylpyruvate pathway. The enzymes Kdc4427 and Adh4428 from Enterobacter sp. CGMCC 5087 showed higher performances than did the corresponding enzymes ARO10 and ADH2 from Saccharomyces cerevisiae, respectively. The E. coli cell factory was further improved by overexpressing two upstream shikimate pathway genes, aroF/aroG/aroH and pheA, to enhance the metabolic flux of the phenylpyruvate pathway, which resulted in 2-PE production of 260mg/L. The combined overexpression of tktA and ppsA increased the precursor supply of erythrose-4-phosphate and phosphoenolpyruvate, which resulted in 2-PE production of 320mg/L, with a productivity of 13.3mg/L/h.ConclusionsThe present study achieved the highest titer of de novo 2-PE production of in a recombinant E. coli system. This study describes a new, efficient 2-PE producer that lays foundation for the industrial-scale production of 2-PE and its derivatives in the future.
Keyword2-Phenylethanol Enterobacter sp CGMCC 5087 2-Keto acid decarboxylase Alcohol dehydrogenase Phenylpyruvate pathway Metabolic engineering
DOI10.1186/s13068-018-1297-3
WOS KeywordSACCHAROMYCES-CEREVISIAE ; EHRLICH PATHWAY ; L-PHENYLALANINE ; PHENYLPYRUVATE DECARBOXYLASE ; PHENYLACETALDEHYDE SYNTHASE ; BIOTECHNOLOGICAL PRODUCTION ; METABOLISM ; BIOFUELS ; YEAST ; BIOCATALYSTS
Language英语
Funding ProjectNational Natural Science Foundation of China (NSF)[31400084] ; Hainan's Key Project of Research and Development Plan[ZDYF2017155] ; Taishan Scholars Climbing Program of Shandong[TSPD20150210] ; Youth Innovation Promotion Association CAS[2017252]
WOS Research AreaBiotechnology & Applied Microbiology ; Energy & Fuels
Funding OrganizationNational Natural Science Foundation of China (NSF) ; Hainan's Key Project of Research and Development Plan ; Taishan Scholars Climbing Program of Shandong ; Youth Innovation Promotion Association CAS
WOS SubjectBiotechnology & Applied Microbiology ; Energy & Fuels
WOS IDWOS:000449850300004
PublisherBMC
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Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/10999
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorXian, Mo; Zhang, Haibo
Affiliation1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Agr Sci, Key Lab Tobacco Gene Resources, Tobacco Res Inst, Qingdao 266101, Peoples R China
Recommended Citation
GB/T 7714
Liu, Changqing,Zhang, Kai,Cao, Wenyan,et al. Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli[J]. BIOTECHNOLOGY FOR BIOFUELS,2018,11:15.
APA Liu, Changqing.,Zhang, Kai.,Cao, Wenyan.,Zhang, Ge.,Chen, Guoqiang.,...&Zhang, Haibo.(2018).Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli.BIOTECHNOLOGY FOR BIOFUELS,11,15.
MLA Liu, Changqing,et al."Genome mining of 2-phenylethanol biosynthetic genes from Enterobacter sp CGMCC 5087 and heterologous overproduction in Escherichia coli".BIOTECHNOLOGY FOR BIOFUELS 11(2018):15.
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