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Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants
Fu, Huihui1,2,3; Liu, Lulu1,2; Dong, Ziyang1,2; Guo, Shupan1,2; Gao, Haichun1,2
2018-04-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume84Issue:8
AbstractIron, a major protein cofactor, is essential for most organisms but can simultaneously be toxic. Iron homeostasis thus has to be effectively maintained under a range of iron regimes. This may be particularly true with Shewanella oneidensis, a representative of dissimilatory metal-reducing bacteria (DMRB), which are capable of respiring a variety of chemicals as electron acceptors (EAs), including iron ores. Although iron respiration and its regulation have been extensively studied in this bacterium, how iron homeostasis is maintained remains largely unknown. Here, we report that the loss of the iron homeostasis master regulator Fur negatively affects the respiration of all EAs tested. This defect appears mainly to be a result of reduced cytochrome c (cyt c) production, despite a decrease in the expression of reductases that are under the direct control of Fur. We also show that S. oneidensis Fur interacts with canonical Fur box motifs in F-F-x-R configuration rather than the palindromic motif proposed before. The fur mutant has lowered total iron and increased free iron contents. Under iron-rich conditions, overproduction of the major iron storage protein Bfr elevates the total iron levels of the fur mutant over those of the wildtype but does not affect free iron levels. Intriguingly, such an operation only marginally improves cyt c production by affecting heme b biosynthesis. It is established that iron dictates heme b/cyt c biosynthesis in S. oneidensis fur(+) strains, but the fur mutation annuls the dependence of heme b/cyt c biosynthesis on iron. Overall, our results suggest that Fur has a profound impact on the iron homeostasis of S. oneidensis, through which many physiological processes, especially respiration, are transformed.
SubtypeArticle
KeywordIron Homeostasis Shewanella Fur Regulation Respiration Cytochrome c
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1128/AEM.00039-18
WOS KeywordANAEROBIC RESPIRATION ; OXIDATIVE STRESS ; ESCHERICHIA-COLI ; CYTOCHROME ; METABOLISM ; MR-1 ; MECHANISMS ; REGULATOR ; PROTEIN ; INVOLVEMENT
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
Funding OrganizationNational Natural Science Foundation of China(41476105) ; Natural Science Foundation of Zhejiang Province(LZ17C010001)
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000429665700001
PublisherAMER SOC MICROBIOLOGY
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/10826
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorGao, Haichun
Affiliation1.Zhejiang Univ, Inst Microbiol, Hangzhou, Zhejiang, Peoples R China
2.Zhejiang Univ, Coll Life Sci, Hangzhou, Zhejiang, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China
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Fu, Huihui,Liu, Lulu,Dong, Ziyang,et al. Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(8).
APA Fu, Huihui,Liu, Lulu,Dong, Ziyang,Guo, Shupan,&Gao, Haichun.(2018).Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(8).
MLA Fu, Huihui,et al."Dissociation between Iron and Heme Biosyntheses Is Largely Accountable for Respiration Defects of Shewanella oneidensis fur Mutants".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.8(2018).
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