QIBEBT-IR  > 单细胞中心组群
Saliva microbiomes distinguish caries-active from healthy human populations
Yang, Fang1,2,3; Zeng, Xiaowei4; Ning, Kang4; Liu, Kuan-Liang5; Lo, Chien-Chi5; Wang, Wei4; Chen, Jie4; Wang, Dongmei4; Huang, Ranran4; Chang, Xingzhi4; Chain, Patrick S.5; Xie, Gary5; Ling, Junqi1,2,3; JianXu4
2012
Source PublicationISME JOURNAL
Volume6Issue:1Pages:1-10
Abstract

The etiology of dental caries remains elusive because of our limited understanding of the complex oral microbiomes. The current methodologies have been limited by insufficient depth and breadth of microbial sampling, paucity of data for diseased hosts particularly at the population level, inconsistency of sampled sites and the inability to distinguish the underlying microbial factors. By cross-validating 16S rRNA gene amplicon-based and whole-genome-based deep-sequencing technologies, we report the most in-depth, comprehensive and collaborated view to date of the adult saliva microbiomes in pilot populations of 19 caries-active and 26 healthy human hosts. We found that: first, saliva microbiomes in human population were featured by a vast phylogenetic diversity yet a minimal organismal core; second, caries microbiomes were significantly more variable in community structure whereas the healthy ones were relatively conserved; third, abundance changes of certain taxa such as overabundance of Prevotella Genus distinguished caries microbiota from healthy ones, and furthermore, caries-active and normal individuals carried different arrays of Prevotella species; and finally, no 'caries-specific' operational taxonomic units (OTUs) were detected, yet 147 OTUs were 'caries associated', that is, differentially distributed yet present in both healthy and caries-active populations. These findings underscored the necessity of species- and strain-level resolution for caries prognosis, and were consistent with the ecological hypothesis where the shifts in community structure, instead of the presence or absence of particular groups of microbes, underlie the cariogenesis.

; The etiology of dental caries remains elusive because of our limited understanding of the complex oral microbiomes. The current methodologies have been limited by insufficient depth and breadth of microbial sampling, paucity of data for diseased hosts particularly at the population level, inconsistency of sampled sites and the inability to distinguish the underlying microbial factors. By cross-validating 16S rRNA gene amplicon-based and whole-genome-based deep-sequencing technologies, we report the most in-depth, comprehensive and collaborated view to date of the adult saliva microbiomes in pilot populations of 19 caries-active and 26 healthy human hosts. We found that: first, saliva microbiomes in human population were featured by a vast phylogenetic diversity yet a minimal organismal core; second, caries microbiomes were significantly more variable in community structure whereas the healthy ones were relatively conserved; third, abundance changes of certain taxa such as overabundance of Prevotella Genus distinguished caries microbiota from healthy ones, and furthermore, caries-active and normal individuals carried different arrays of Prevotella species; and finally, no 'caries-specific' operational taxonomic units (OTUs) were detected, yet 147 OTUs were 'caries associated', that is, differentially distributed yet present in both healthy and caries-active populations. These findings underscored the necessity of species-and strain-level resolution for caries prognosis, and were consistent with the ecological hypothesis where the shifts in community structure, instead of the presence or absence of particular groups of microbes, underlie the cariogenesis. The ISME Journal (2012) 6, 1-10; doi:10.1038/ismej.2011.71; published online 30 June 2011
SubtypeArticle
KeywordCaries Metagenomics Oral-microbiome Prevotella Saliva
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1038/ismej.2011.71
WOS KeywordCHILDHOOD CARIES ; ORAL MICROFLORA ; DENTAL-CARIES ; HUMAN-BODY ; COMMUNITY ; DIVERSITY ; CHILDREN ; BACTERIA ; DISEASES ; SYSTEM
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Microbiology
WOS SubjectEcology ; Microbiology
WOS IDWOS:000299052500001
Citation statistics
Cited Times:154[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/1081
Collection单细胞中心组群
Affiliation1.Sun Yat Sen Univ, Dept Operat Dent & Endodont, Guanghua Sch, Guangzhou 510155, Guangdong, Peoples R China
2.Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510155, Guangdong, Peoples R China
3.Sun Yat Sen Univ, Inst Stomatol Res, Guangzhou 510155, Guangdong, Peoples R China
4.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao, Shandong, Peoples R China
5.Los Alamos Natl Lab, Biosci Div, Los Alamos, NM USA
Recommended Citation
GB/T 7714
Yang, Fang,Zeng, Xiaowei,Ning, Kang,et al. Saliva microbiomes distinguish caries-active from healthy human populations[J]. ISME JOURNAL,2012,6(1):1-10.
APA Yang, Fang.,Zeng, Xiaowei.,Ning, Kang.,Liu, Kuan-Liang.,Lo, Chien-Chi.,...&JianXu.(2012).Saliva microbiomes distinguish caries-active from healthy human populations.ISME JOURNAL,6(1),1-10.
MLA Yang, Fang,et al."Saliva microbiomes distinguish caries-active from healthy human populations".ISME JOURNAL 6.1(2012):1-10.
Files in This Item: Download All
File Name/Size DocType Version Access License
Saliva microbiomes d(527KB) 开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yang, Fang]'s Articles
[Zeng, Xiaowei]'s Articles
[Ning, Kang]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yang, Fang]'s Articles
[Zeng, Xiaowei]'s Articles
[Ning, Kang]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yang, Fang]'s Articles
[Zeng, Xiaowei]'s Articles
[Ning, Kang]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Saliva microbiomes distinguish caries-active from healthy human populations.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.