Description of a Zostera marina catalase gene involved in responses to temperature stress

doi:10.7717/peerj.4532

QIBEBT-IR

	Description of a Zostera marina catalase gene involved in responses to temperature stress
	Zang, Yu 1; Liu, Jiao 3; Tang, Xue Xi 1,2; Zhou, Bin 1,2
	2018-03-26
发表期刊	PEERJ
ISSN	2167-8359
卷号	6
摘要	Catalase (CAT) is an antioxidant enzyme that plays a significant role in cellular protection against oxidative darnage by degradation of hydrogen peroxide to oxygen and water. In the present study, the complete CAT cDNA sequence of Zostera marina was identified through expressed sequence tags (EST) analysis and the rapid amplification of cDNA ends (RACE) technique. The nucleotide sequence of ZmCAT cDNA consisted of 1,816 by with a 1,434 bp open reading frame (ORF) encoding a polypeptide of 477 amino acid residues, which possessed significant homology to other known plant CATs. The molecular mass of the predicted protein was 55.3 kDa with an estimated isoelectric point of 6.40. Phylogenetic analysis showed that ZmCAT was closely related to CAT from gramineous species. In response to temperature stress, H2O2 and MDA contents in Z. marina-increased significantly with cold stress (<10 degrees C) and heat stress (>25 degrees C). ZmCAT expression was significantly upregulated at temperatures from 5 to 10 degrees C and then gradually downregulated, reaching its lowest expression at 30 degrees C. Recombinant ZmCAT protein exhibited strong antioxidant activity over a wide temperature range, with the highest rZmCAT activity observed at 25 degrees C and a higher relative activity retained even with heat stress. All these results indicated that ZmCAT was a member of the plant CAT family and involved in minimizing oxidative damage effects in Z. marina under temperature stress.
文章类型	Article
关键词	Cat Temperature Stress Mrna Expression Enzyme Activity
WOS标题词	Science & Technology
DOI	10.7717/peerj.4532
关键词[WOS]	OXIDATIVE STRESS ; HEAT-STRESS ; SEAGRASSES ; TOLERANCE ; EXPRESSION ; L. ; MECHANISMS ; ENZYMES ; GROWTH ; USA
收录类别	SCI
语种	英语
WOS研究方向	Science & Technology - Other Topics
项目资助者	National Natural Science Foundation of China(41476091) ; Key Special Program on "Marine Environmental Security Protection'' of National Research and Development Project of China(2016YFC1402305)
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000428696100002
出版者	PEERJ INC
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10818
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zhou, Bin
作者单位	1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zang, Yu,Liu, Jiao,Tang, Xue Xi,et al. Description of a Zostera marina catalase gene involved in responses to temperature stress[J]. PEERJ,2018,6.
APA	Zang, Yu,Liu, Jiao,Tang, Xue Xi,&Zhou, Bin.(2018).Description of a Zostera marina catalase gene involved in responses to temperature stress.PEERJ,6.
MLA	Zang, Yu,et al."Description of a Zostera marina catalase gene involved in responses to temperature stress".PEERJ 6(2018).