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Description of a Zostera marina catalase gene involved in responses to temperature stress
Zang, Yu1; Liu, Jiao3; Tang, Xue Xi1,2; Zhou, Bin1,2
2018-03-26
Source PublicationPEERJ
ISSN2167-8359
Volume6
AbstractCatalase (CAT) is an antioxidant enzyme that plays a significant role in cellular protection against oxidative darnage by degradation of hydrogen peroxide to oxygen and water. In the present study, the complete CAT cDNA sequence of Zostera marina was identified through expressed sequence tags (EST) analysis and the rapid amplification of cDNA ends (RACE) technique. The nucleotide sequence of ZmCAT cDNA consisted of 1,816 by with a 1,434 bp open reading frame (ORF) encoding a polypeptide of 477 amino acid residues, which possessed significant homology to other known plant CATs. The molecular mass of the predicted protein was 55.3 kDa with an estimated isoelectric point of 6.40. Phylogenetic analysis showed that ZmCAT was closely related to CAT from gramineous species. In response to temperature stress, H2O2 and MDA contents in Z. marina-increased significantly with cold stress (<10 degrees C) and heat stress (>25 degrees C). ZmCAT expression was significantly upregulated at temperatures from 5 to 10 degrees C and then gradually downregulated, reaching its lowest expression at 30 degrees C. Recombinant ZmCAT protein exhibited strong antioxidant activity over a wide temperature range, with the highest rZmCAT activity observed at 25 degrees C and a higher relative activity retained even with heat stress. All these results indicated that ZmCAT was a member of the plant CAT family and involved in minimizing oxidative damage effects in Z. marina under temperature stress.
SubtypeArticle
KeywordCat Temperature Stress Mrna Expression Enzyme Activity
WOS HeadingsScience & Technology
DOI10.7717/peerj.4532
WOS KeywordOXIDATIVE STRESS ; HEAT-STRESS ; SEAGRASSES ; TOLERANCE ; EXPRESSION ; L. ; MECHANISMS ; ENZYMES ; GROWTH ; USA
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
Funding OrganizationNational Natural Science Foundation of China(41476091) ; Key Special Program on "Marine Environmental Security Protection'' of National Research and Development Project of China(2016YFC1402305)
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000428696100002
PublisherPEERJ INC
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/10818
Collection中国科学院青岛生物能源与过程研究所
Corresponding AuthorZhou, Bin
Affiliation1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China
Recommended Citation
GB/T 7714
Zang, Yu,Liu, Jiao,Tang, Xue Xi,et al. Description of a Zostera marina catalase gene involved in responses to temperature stress[J]. PEERJ,2018,6.
APA Zang, Yu,Liu, Jiao,Tang, Xue Xi,&Zhou, Bin.(2018).Description of a Zostera marina catalase gene involved in responses to temperature stress.PEERJ,6.
MLA Zang, Yu,et al."Description of a Zostera marina catalase gene involved in responses to temperature stress".PEERJ 6(2018).
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