Naphthylacetic Acid and Tea Polyphenol Application Promote Biomass and Lipid Production of Nervonic Acid-Producing Microalgae

doi:10.3389/fpls.2018.00506

QIBEBT-IR

	Naphthylacetic Acid and Tea Polyphenol Application Promote Biomass and Lipid Production of Nervonic Acid-Producing Microalgae
	Xu, Feng 1,2; Fan, Yong 2; Miao, Fuhong 1; Hu, Guang-Rong 2; Sun, Juan 1; Yang, Guofeng 1; Li, Fu-Li 2
	2018-04-17
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	9
摘要	Mychonastes afer HSO-3-1 is a potential producer of nervonic acid, which could be accumulated to 2-3% of dry cell weight. Improving the productivity of nervonic acid is critical to promote the commercialization of this product. In this study, 1-naphthylacetic acid (NAA) and tea polyphenol (TP) were selected as bioactive additives to stimulate the growth of M. afer. Supplementing NAA in the early growth stage and TP in the middle and late growth stage led to improved lipid accumulation in M. afer. The cultures supplemented with TP at the late growth stage maintained higher photosynthetic efficiency than the control groups without TP. Furthermore, the intracellular reactive oxygen species (ROS) accumulations in M. afer supplemented with 500 mg/L of TP was 63% lower than the control group. A linear relationship (R-2 = 0.899) between the values of Fv/Fm and ROS accumulation was established. We hypothesize supplement of bioactive additives at different growth stage could promote the cell growth rate and nervonic acid productivity of M. afer by retrieving intracellular ROS level. Further analysis of photosynthetic system II (PSII) protein in M. afer cultured in presence of NAA and TP indicated the levels of D1 and D2 proteins, the core skeleton proteins of PSII, showed 33.3 and 25.6% higher than the control group. CP43 protein, a critical module in PSII repair cycle, decreased significantly. These implied that TP possesses the function of slowing down the damage of PSII by scavenging excess intracellular ROS.
文章类型	Article
关键词	Mychonastes Afer Photosynthetic Efficiency Reactive Oxygen Species Photosynthetic System Ii Cp43 Bioactive Additive
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fpls.2018.00506
关键词[WOS]	CHLAMYDOMONAS-REINHARDTII ; OXIDATIVE STRESS ; PHOTOSYSTEM-II ; TOLERANCE ; BIODIESEL ; PROTEIN ; ALGAE ; PERSPECTIVES ; CHLOROPLAST ; FEEDSTOCKS
收录类别	SCI
语种	英语
WOS研究方向	Plant Sciences
项目资助者	China Agriculture Research System(CARS-34) ; Forage Industrial Innovation Team, Shandong Modern Agricultural Industrial and Technical System(SDAIT-23-01) ; National Natural Science Foundation of China(31602154)
WOS类目	Plant Sciences
WOS记录号	WOS:000430199900001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10814
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Yang, Guofeng
作者单位	1.Qingdao Agr Univ, Forage Res & Dev Ctr Arable Reg, Qingdao, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Xu, Feng,Fan, Yong,Miao, Fuhong,et al. Naphthylacetic Acid and Tea Polyphenol Application Promote Biomass and Lipid Production of Nervonic Acid-Producing Microalgae[J]. FRONTIERS IN PLANT SCIENCE,2018,9.
APA	Xu, Feng.,Fan, Yong.,Miao, Fuhong.,Hu, Guang-Rong.,Sun, Juan.,...&Li, Fu-Li.(2018).Naphthylacetic Acid and Tea Polyphenol Application Promote Biomass and Lipid Production of Nervonic Acid-Producing Microalgae.FRONTIERS IN PLANT SCIENCE,9.
MLA	Xu, Feng,et al."Naphthylacetic Acid and Tea Polyphenol Application Promote Biomass and Lipid Production of Nervonic Acid-Producing Microalgae".FRONTIERS IN PLANT SCIENCE 9(2018).