KMS Qingdao Institute of Biomass Energy and Bioprocess Technology ,CAS
Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts | |
Zhang, Ning1,2; Fan, Yuxuan1,2; Li, Chen3,4; Wang, Qiming5; Leksawasdi, Noppol6; Li, Fuli1; Wang, Shi'an1 | |
2018-05-01 | |
发表期刊 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY |
ISSN | 0175-7598 |
卷号 | 102期号:9页码:4183-4191 |
摘要 | Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether the classical nuclear DNA staining method for ascomycetous yeasts is applicable to basidiomycetous yeasts. In this study, 5 yeasts ineffectively stained by the classical propidium iodide (PI) staining method were identified from 23 representative basidiomycetous yeasts. Pretreatment of cells using dimethyl sulfoxide (DMSO) or snailase markedly improved cell penetration to PI and thus enabled DNA content determination by flow cytometry on the recalcitrant yeasts. The pretreatments are efficient, simple, and fast, avoiding tedious mutagenesis or genetic engineering used in previous reports. The heterogeneity of cell penetration to PI among basidiomycetous yeasts was attributed to the discrepancy in cell wall polysaccharides instead of capsule or plasma membrane. This study also indicated that care must be taken in attributing PI-negative staining as viable cells when studying non-model microorganisms. |
文章类型 | Article |
关键词 | Basidiomycetous Yeast Cell Permeability Cell Wall Dimethyl Sulfoxide Dna Staining |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1007/s00253-018-8906-8 |
关键词[WOS] | SACCHAROMYCES-CEREVISIAE ; PHAFFIA-RHODOZYMA ; FLOW-CYTOMETRY ; DIMETHYL-SULFOXIDE ; PHYLOGENETIC CLASSIFICATION ; CRYPTOCOCCUS-NEOFORMANS ; PENETRATION ENHANCERS ; ESCHERICHIA-COLI ; WALL ; VIABILITY |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biotechnology & Applied Microbiology |
项目资助者 | Natural Science Foundation of China(31670054 ; Youth Innovation Promotion Association, Chinese Academy of Sciences(2013137) ; 51561145015 ; 21676159) |
WOS类目 | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000429800600026 |
出版者 | SPRINGER |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/10768 |
专题 | 中国科学院青岛生物能源与过程研究所 |
通讯作者 | Wang, Shi'an |
作者单位 | 1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao Engn Lab Single Cell Oil, Key Lab Biofuels,Qingdao Inst Bioenergy & Bioproc, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Key Lab Maricultural Organism Dis Control, Minist Agr,Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China 6.Chiang Mai Univ, Sch Agroind, Chiang Mai 50100, Thailand |
推荐引用方式 GB/T 7714 | Zhang, Ning,Fan, Yuxuan,Li, Chen,et al. Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(9):4183-4191. |
APA | Zhang, Ning.,Fan, Yuxuan.,Li, Chen.,Wang, Qiming.,Leksawasdi, Noppol.,...&Wang, Shi'an.(2018).Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(9),4183-4191. |
MLA | Zhang, Ning,et al."Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.9(2018):4183-4191. |
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