Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts

doi:10.1007/s00253-018-8906-8

QIBEBT-IR

	Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts
	Zhang, Ning 1,2; Fan, Yuxuan 1,2; Li, Chen 3,4; Wang, Qiming 5; Leksawasdi, Noppol 6; Li, Fuli1 ; Wang, Shi'an 1
	2018-05-01
发表期刊	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN	0175-7598
卷号	102 期号:9 页码:4183-4191
摘要	Non-model yeasts within basidiomycetes have considerable importance in agriculture, industry, and environment, but they are not as well studied as ascomycetous yeasts. Serving as a basic technique, nuclear DNA staining is widely used in physiology, ecology, cell biology, and genetics. However, it is unclear whether the classical nuclear DNA staining method for ascomycetous yeasts is applicable to basidiomycetous yeasts. In this study, 5 yeasts ineffectively stained by the classical propidium iodide (PI) staining method were identified from 23 representative basidiomycetous yeasts. Pretreatment of cells using dimethyl sulfoxide (DMSO) or snailase markedly improved cell penetration to PI and thus enabled DNA content determination by flow cytometry on the recalcitrant yeasts. The pretreatments are efficient, simple, and fast, avoiding tedious mutagenesis or genetic engineering used in previous reports. The heterogeneity of cell penetration to PI among basidiomycetous yeasts was attributed to the discrepancy in cell wall polysaccharides instead of capsule or plasma membrane. This study also indicated that care must be taken in attributing PI-negative staining as viable cells when studying non-model microorganisms.
文章类型	Article
关键词	Basidiomycetous Yeast Cell Permeability Cell Wall Dimethyl Sulfoxide Dna Staining
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1007/s00253-018-8906-8
关键词[WOS]	SACCHAROMYCES-CEREVISIAE ; PHAFFIA-RHODOZYMA ; FLOW-CYTOMETRY ; DIMETHYL-SULFOXIDE ; PHYLOGENETIC CLASSIFICATION ; CRYPTOCOCCUS-NEOFORMANS ; PENETRATION ENHANCERS ; ESCHERICHIA-COLI ; WALL ; VIABILITY
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	Natural Science Foundation of China(31670054 ; Youth Innovation Promotion Association, Chinese Academy of Sciences(2013137) ; 51561145015 ; 21676159)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000429800600026
出版者	SPRINGER
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10768
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Wang, Shi'an
作者单位	1.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao Engn Lab Single Cell Oil, Key Lab Biofuels,Qingdao Inst Bioenergy & Bioproc, 189 Songling Rd, Qingdao 266101, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Chinese Acad Fishery Sci, Qingdao Key Lab Mariculture Epidemiol & Biosecur, Key Lab Maricultural Organism Dis Control, Minist Agr,Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Funct Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266071, Peoples R China 5.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China 6.Chiang Mai Univ, Sch Agroind, Chiang Mai 50100, Thailand
推荐引用方式 GB/T 7714	Zhang, Ning,Fan, Yuxuan,Li, Chen,et al. Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2018,102(9):4183-4191.
APA	Zhang, Ning.,Fan, Yuxuan.,Li, Chen.,Wang, Qiming.,Leksawasdi, Noppol.,...&Wang, Shi'an.(2018).Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,102(9),4183-4191.
MLA	Zhang, Ning,et al."Cell permeability and nuclear DNA staining by propidium iodide in basidiomycetous yeasts".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 102.9(2018):4183-4191.