Oxidation of organic electron donor by denitratation: Performance, pathway and key microorganism

doi:10.1016/j.cej.2018.02.112

QIBEBT-IR

	Oxidation of organic electron donor by denitratation: Performance, pathway and key microorganism
	Li, Wei 1,2,6; Liu, Shuai 3; Zhang, Meng 1; Zhao, He-Ping 1,4; Zheng, Ping 1,4,5
	2018-07-01
发表期刊	CHEMICAL ENGINEERING JOURNAL
ISSN	1385-8947
卷号	343 页码:554-560
摘要	In this paper, two classical organic electron donors, i.e. acetate and glucose, were chosen to study the oxidation of organics coupling with denitratation enrichment culture via batch tests and to predicate its key functional bacteria via genomes-based metagenomics. The results showed that acetate-denitratation was observed to have better denitratation performances (nitrate reduction rate of 13.5-18.5 mg.g(-1)VSS.h(-1) and nitrite yield of 96.2%) than glucose-denitratation (nitrate reduction rate of 4.6-6.5 mg.g(-1)VSS.h(-1) and nitrite yield of 71.5%). Distinctive but preferable acetogenesis (acetate production) step was observed in glucose-fed test, which resulted in the decline of denitratation performance by lower intracellular NADH/NAD(+) ratio. The enzymatic assay of key carbon metabolism indicated that the low NADH/NAD(+) ratio was likely impacted by up-regulating the acetate generation from glucose and down-regulating the acetate utilization. Metagenomic binning and phylogenetic analysis revealed that a new Halomonas genome (only shared an average nucleotide identify of 83% to the most related species) dominated in the system, and was most likely responsible for this kind of acetogenesis in denitratation. The findings of this work are important for both the development of denitratation-based nitrogen removal technology and the insight into fundamental microbial metabolism of nitrogen coupling carbon oxidation.
文章类型	Article
关键词	Nitrogen Bioremoval Organic Electron Donor Denitratation Enzymatic Assay Metagenomics
WOS标题词	Science & Technology ; Technology
DOI	10.1016/j.cej.2018.02.112
关键词[WOS]	WASTE-WATER TREATMENT ; ANAEROBIC AMMONIUM OXIDATION ; NITRATE REDUCTION ; NITROGEN REMOVAL ; DENITRIFICATION ; BACTERIA ; ANAMMOX ; ENRICHMENT ; CULTURE ; REACTOR
收录类别	SCI
语种	英语
WOS研究方向	Engineering
项目资助者	Natural Science Foundation of China(51578484 ; China Postdoctoral Science Foundation China(2016M590540) ; Fundamental Research Funds for the Central Universities(222201814053) ; Research Fund Program of Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology(2018K16) ; 51608474 ; 41601243)
WOS类目	Engineering, Environmental ; Engineering, Chemical
WOS记录号	WOS:000430269200058
出版者	ELSEVIER SCIENCE SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10754
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Zheng, Ping
作者单位	1.Zhejiang Univ, Coll Environm & Resource Sci, Dept Environm Engn, Hangzhou, Zhejiang, Peoples R China 2.East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Beijing, Peoples R China 4.Zhejiang Univ, Zhejiang Prov Key Lab Water Pollut Control & Envi, Hangzhou, Zhejiang, Peoples R China 5.Zhejiang Univ, Coll Environm & Resource Sci, MOE Key Lab Environm Remediat & Ecosyst Hlth, Hangzhou, Zhejiang, Peoples R China 6.Sun Yat Sen Univ, Guangdong Prov Key Lab Environm Pollut Control &, Guangzhou, Guangdong, Peoples R China
推荐引用方式 GB/T 7714	Li, Wei,Liu, Shuai,Zhang, Meng,et al. Oxidation of organic electron donor by denitratation: Performance, pathway and key microorganism[J]. CHEMICAL ENGINEERING JOURNAL,2018,343:554-560.
APA	Li, Wei,Liu, Shuai,Zhang, Meng,Zhao, He-Ping,&Zheng, Ping.(2018).Oxidation of organic electron donor by denitratation: Performance, pathway and key microorganism.CHEMICAL ENGINEERING JOURNAL,343,554-560.
MLA	Li, Wei,et al."Oxidation of organic electron donor by denitratation: Performance, pathway and key microorganism".CHEMICAL ENGINEERING JOURNAL 343(2018):554-560.