A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency

doi:10.1016/j.snb.2018.01.077

QIBEBT-IR

	A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency
	Wang, Xixian 1,2,3; Ge, Anle 1; Hu, Liang 1; Feng, Xiaojun 1; Du, Wei 1; Liu, Bi-Feng 1
	2018-05-01
发表期刊	SENSORS AND ACTUATORS B-CHEMICAL
ISSN	0925-4005
卷号	260 页码:311-319
摘要	C. elegans is a leading model organism for addressing many fundamental biology issues. Sufficient quantities of the worms with specific developmental stage are needed in C. elegans studies due to the size-dependent features shown in C. elegans. In this work, we presented a simple and feasible microfluidic chip to realize simultaneous sorting of C. elegans with different sizes by graduated microfilters. The micro-device was fabricated with PDMS-agarose hybrid layers consisting of 5 arrays of geometrically optimized square columns as microfilters for separating worms of different sizes. Fluid flow and electric field were simultaneously applied to drive the worms to rapidly and directionally pass through the microfilters, resulting in increased sorting efficiency and accuracy. Further, the sorted worms could be easily collected from 4 grooves of the agarose layer by slipping the PDMS layer to the top of grooves. With this sorting strategy, we demonstrated the feasibility of simultaneously sorting different sizes of C. elegans with high efficiency and accuracy (>95%) and throughput of hundreds of worms per minute. Therefore, the micro-device could be a promising tool to C. elegans assays that need a large number of stage-synchronized worms. It could also be useful for body size-based mutant screening and taxis-response studies in C. elegans. (c) 2018 Elsevier B.V. All rights reserved.
文章类型	Article
关键词	Microfluidic Chip C. Elegans Size-dependent Sorting Electrotaxis Microfilters
WOS标题词	Science & Technology ; Physical Sciences ; Technology
DOI	10.1016/j.snb.2018.01.077
关键词[WOS]	NEMATODE CAENORHABDITIS-ELEGANS ; HIGH-THROUGHPUT ; IN-VIVO ; NEURONAL-ACTIVITY ; SYSTEM ; DEVICE ; PLATFORM ; MOTILITY ; SCREENS
收录类别	SCI
语种	英语
WOS研究方向	Chemistry ; Electrochemistry ; Instruments & Instrumentation
项目资助者	National Key R&D Program of China(2016YFF0100801) ; National Natural Science Foundation of China(21475049 ; Fundamental Research Funds for the Central Universities(2016YXZD061) ; NIH National Centre for Research Resources ; 31471257 ; 21275060)
WOS类目	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
WOS记录号	WOS:000424884300037
出版者	ELSEVIER SCIENCE SA
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10748
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Du, Wei; Liu, Bi-Feng
作者单位	1.Huazhong Univ Sci & Technol, Coll Life Sci & Technol,Key Lab Biomed Photon, Dept Biomed Engn,Wuhan Natl Lab Optoelect,MOE, Syst Biol Theme,Hubei Bioinformat & Mol Imaging K, Wuhan 430074, Hubei, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xixian,Ge, Anle,Hu, Liang,et al. A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency[J]. SENSORS AND ACTUATORS B-CHEMICAL,2018,260:311-319.
APA	Wang, Xixian,Ge, Anle,Hu, Liang,Feng, Xiaojun,Du, Wei,&Liu, Bi-Feng.(2018).A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency.SENSORS AND ACTUATORS B-CHEMICAL,260,311-319.
MLA	Wang, Xixian,et al."A microfluidic microfilter chip driven by electrotaxis and fluid flow for size-dependent C-elegans sorting with high purity and efficiency".SENSORS AND ACTUATORS B-CHEMICAL 260(2018):311-319.