Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress

doi:10.3389/fmicb.2018.00682

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	Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress
	Zheng, Rikuan 1,2,3; Wu, Shimei 4; Ma, Ning 1,2,3; Sun, Chaomin 1,2
	2018-04-05
发表期刊	FRONTIERS IN MICROBIOLOGY
ISSN	1664-302X
卷号	9
摘要	Mercury-mediated toxicity remains one of the greatest barriers against microbial survival, even though bacterial resistance to mercury compounds can occur. However, the genetic and physiological adaptations of bacteria to mercury stress still remains unclear. Here, we show that the marine bacterium Pseudomonas stutzeri 273 is resistant to 50 mu M Hg2+ and removes up to 94% Hg2+ from culture. Using gene homologous recombination and complementation, we show that genes encoding Hg2+-transport proteins MerT, MerP, the mercuric reductase MerA and the regulatory protein MerD are essential for bacterial mercuric resistance when challenged with Hg2+. Further, mercury stress inhibits flagellar development, motility, chemotaxis and biofilm formation of P. stutzeri 273, which are verified by transcriptomic and physiological analyses. Surprisingly, we discover that MerF, a previously reported Hg2+-transporter, determines flagellar development, motility and biofilm formation in P. stutzeri 273 by genetic and physiological analyses. Our results strongly indicate that MerF plays an integral role in P. stutzeri 273 to develop physiological responses to mercury stress. Notably, MerF homologs are also prevalent in different human pathogens. Using this unique target may provide novel strategies to control these pathogenic bacteria, given the role of MerF in flagella and biofilm formation. In summary, our data provide an original report on MerF in bacterial physiological development and suggest that the mer in marine bacteria has evolved through progressive, sequential recruitment of novel functions over time.
文章类型	Article
关键词	Marine Pseudomonas Stutzeri Mercury Stress Motility Flagella
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.3389/fmicb.2018.00682
关键词[WOS]	ESCHERICHIA-COLI ; SWARMING MOTILITY ; RESISTANCE OPERON ; MER OPERON ; PROTEIN ; TRANSPORT ; BIOREMEDIATION ; DETOXIFICATION ; PRECIPITATION ; MECHANISM
收录类别	SCI
语种	英语
WOS研究方向	Microbiology
项目资助者	Taishan Young Scholar Foundation of Shandong Province(tsqn20161051) ; Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; Natural Science Outstanding Youth Fund of Shandong Province(JQ201607) ; "100-Talent Project" of the Chinese Academy of Sciences
WOS类目	Microbiology
WOS记录号	WOS:000429289200001
出版者	FRONTIERS MEDIA SA
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10728
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Sun, Chaomin
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China 4.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Zheng, Rikuan,Wu, Shimei,Ma, Ning,et al. Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress[J]. FRONTIERS IN MICROBIOLOGY,2018,9.
APA	Zheng, Rikuan,Wu, Shimei,Ma, Ning,&Sun, Chaomin.(2018).Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress.FRONTIERS IN MICROBIOLOGY,9.
MLA	Zheng, Rikuan,et al."Genetic and Physiological Adaptations of Marine Bacterium Pseudomonas stutzeri 273 to Mercury Stress".FRONTIERS IN MICROBIOLOGY 9(2018).