QIBEBT-IR  > 分子微生物工程研究组
Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer
Fan, Yong1; Yuan, Cheng2; Jin, Yi1; Hu, Guang-Rong2; Li, Fu-Li1
2018-04-01
Source PublicationALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
Volume31Pages:225-231
AbstractNervonic acid (C24:1, Delta 15), a monounsaturated long chain fatty acid, is a major component of mammalian neural tissue. Lack of nervonic acid in human usually leads to neurological disorder. Recently, production of nervonic acid from plant for pharmaceutical, nutraceutical, and functional food received increasing attention. We have previously isolated a microalgal strain Mychonastes afer HSO-3-1, which can accumulate approximately 6% of nervonic acid and <0.5% of erucic acid in triacylglycerol. In this study, the full length of 3-ketoacyl-coA synthase gene MaKCS, encoding the first component of fatty acid elongation complex, was cloned and expressed in Saccharomyces cerevisiae BY4741 under the control of GAL1 promoter. S. cerevisiae harboring pYC230-Gal-MaKCS resulted in the production of 1.5% nervonic acid in total lipid. The expression of KCS gene in M. afer was found to be induced under stress conditions such as nitrogen deficiency and high light, which was revealed by real-time PCR analysis. MaKCS was demonstrated to be able to extend very long chain monounsaturated fatty acids including nervonic acid, which provides insights into how nervonic acid is synthesized in microalgae.
SubtypeArticle
KeywordNervonic Acid 3-ketoacyl-coa Synthase Mychonastes Afer Saccharomyces Cerevisiae
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
DOI10.1016/j.algal.2018.02.017
WOS KeywordKETOACYL-COA SYNTHASE ; CHAIN FATTY-ACIDS ; SUBSTRATE-SPECIFICITY ; FAE1 GENE ; FUNCTIONAL-CHARACTERIZATION ; ARABIDOPSIS-THALIANA ; LUNARIA-ANNUA ; ERUCIC-ACID ; RAT-LIVER ; EXPRESSION
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology
Funding OrganizationMinistry of Science and Technology of China(2014AA022003 ; National Science Foundation of China(U1232126 ; Shandong Province Natural Science Funds for Distinguished Young Scholar(JQ201507) ; 2015BAD15B02) ; 31602154)
WOS SubjectBiotechnology & Applied Microbiology
WOS IDWOS:000428687600024
PublisherELSEVIER SCIENCE BV
Citation statistics
Document Type期刊论文
Identifierhttp://ir.qibebt.ac.cn/handle/337004/10720
Collection分子微生物工程研究组
Corresponding AuthorLi, Fu-Li
Affiliation1.Chinese Acad Sci, Qingdao Engn Lab Single Cell Oil, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266101, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China
Recommended Citation
GB/T 7714
Fan, Yong,Yuan, Cheng,Jin, Yi,et al. Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2018,31:225-231.
APA Fan, Yong,Yuan, Cheng,Jin, Yi,Hu, Guang-Rong,&Li, Fu-Li.(2018).Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,31,225-231.
MLA Fan, Yong,et al."Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 31(2018):225-231.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Fan, Yong]'s Articles
[Yuan, Cheng]'s Articles
[Jin, Yi]'s Articles
Baidu academic
Similar articles in Baidu academic
[Fan, Yong]'s Articles
[Yuan, Cheng]'s Articles
[Jin, Yi]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Fan, Yong]'s Articles
[Yuan, Cheng]'s Articles
[Jin, Yi]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.