Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer

doi:10.1016/j.algal.2018.02.017

QIBEBT-IR > 分子微生物工程研究组

	Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer
	Fan, Yong 1; Yuan, Cheng 2; Jin, Yi 1; Hu, Guang-Rong 2; Li, Fu-Li1
	2018-04-01
发表期刊	ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN	2211-9264
卷号	31 页码:225-231
摘要	Nervonic acid (C24:1, Delta 15), a monounsaturated long chain fatty acid, is a major component of mammalian neural tissue. Lack of nervonic acid in human usually leads to neurological disorder. Recently, production of nervonic acid from plant for pharmaceutical, nutraceutical, and functional food received increasing attention. We have previously isolated a microalgal strain Mychonastes afer HSO-3-1, which can accumulate approximately 6% of nervonic acid and <0.5% of erucic acid in triacylglycerol. In this study, the full length of 3-ketoacyl-coA synthase gene MaKCS, encoding the first component of fatty acid elongation complex, was cloned and expressed in Saccharomyces cerevisiae BY4741 under the control of GAL1 promoter. S. cerevisiae harboring pYC230-Gal-MaKCS resulted in the production of 1.5% nervonic acid in total lipid. The expression of KCS gene in M. afer was found to be induced under stress conditions such as nitrogen deficiency and high light, which was revealed by real-time PCR analysis. MaKCS was demonstrated to be able to extend very long chain monounsaturated fatty acids including nervonic acid, which provides insights into how nervonic acid is synthesized in microalgae.
文章类型	Article
关键词	Nervonic Acid 3-ketoacyl-coa Synthase Mychonastes Afer Saccharomyces Cerevisiae
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1016/j.algal.2018.02.017
关键词[WOS]	KETOACYL-COA SYNTHASE ; CHAIN FATTY-ACIDS ; SUBSTRATE-SPECIFICITY ; FAE1 GENE ; FUNCTIONAL-CHARACTERIZATION ; ARABIDOPSIS-THALIANA ; LUNARIA-ANNUA ; ERUCIC-ACID ; RAT-LIVER ; EXPRESSION
收录类别	SCI
语种	英语
WOS研究方向	Biotechnology & Applied Microbiology
项目资助者	Ministry of Science and Technology of China(2014AA022003 ; National Science Foundation of China(U1232126 ; Shandong Province Natural Science Funds for Distinguished Young Scholar(JQ201507) ; 2015BAD15B02) ; 31602154)
WOS类目	Biotechnology & Applied Microbiology
WOS记录号	WOS:000428687600024
出版者	ELSEVIER SCIENCE BV
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10720
专题	分子微生物工程研究组
通讯作者	Li, Fu-Li
作者单位	1.Chinese Acad Sci, Qingdao Engn Lab Single Cell Oil, Shandong Prov Key Lab Energy Genet, Key Lab Biofuels, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Fan, Yong,Yuan, Cheng,Jin, Yi,et al. Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2018,31:225-231.
APA	Fan, Yong,Yuan, Cheng,Jin, Yi,Hu, Guang-Rong,&Li, Fu-Li.(2018).Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,31,225-231.
MLA	Fan, Yong,et al."Characterization of 3-ketoacyl-coA synthase in a nervonic acid producing oleaginous microalgae Mychonastes afer".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 31(2018):225-231.