MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis

doi:10.1105/tpc.17.00953

QIBEBT-IR

	MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis
	Li, Shengjun 1,2,3; Liu, Kan 2,3; Zhou, Bangjun 2,4; Li, Mu 2,3; Zhang, Shuxin 5; Zeng, Lirong 2,4; Zhang, Chi 2,3; Yu, Bin 2,3
	2018-02-01
发表期刊	PLANT CELL
ISSN	1040-4651
卷号	30 期号:2 页码:481-494
摘要	MAC3A and MAC3B are conserved U-box-containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis thaliana. However, their functional mechanisms remain elusive. Here, we show that Arabidopsis MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs (MIR genes), suggesting that they may not affect MIR transcription. This result, together with the fact that MAC3A associates with primiRNAs in vivo, indicates that MAC3A and MAC3B may stabilize pri-miRNAs. Furthermore, we find that MAC3A and MAC3B interact with the DCL1 complex that catalyzes miRNA maturation, promote DCL1 activity, and are required for the localization of HYL1, a component of the DCL1 complex. Besides MAC3A and MAC3B, two other MAC subunits, CDC5 and PRL1, also function in miRNA biogenesis. Based on these results, we propose that MAC functions as a complex to control miRNA levels through modulating pri-miRNA transcription, processing, and stability.
文章类型	Article
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
DOI	10.1105/tpc.17.00953
关键词[WOS]	PRIMARY MICRORNA TRANSCRIPTS ; TRANS-ACTING SIRNAS ; BINDING PROTEIN ; ARABIDOPSIS-THALIANA ; SMALL RNAS ; PRI-MIRNA ; PLANT MICRORNAS ; CYCLOPHILIN 40 ; POLYMERASE-II ; U-BOX
收录类别	SCI
语种	英语
WOS研究方向	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
项目资助者	Nebraska Soybean Board(1727) ; National Science Foundation(OIA-1557417 ; Pioneer Hundred Talents Program of Chinese Academy of Sciences ; IOS-1645659)
WOS类目	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
WOS记录号	WOS:000427109700023
出版者	AMER SOC PLANT BIOLOGISTS
引用统计
文献类型	期刊论文
条目标识符	http://ir.qibebt.ac.cn/handle/337004/10712
专题	中国科学院青岛生物能源与过程研究所
通讯作者	Yu, Bin
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao 266101, Peoples R China 2.Univ Nebraska, Ctr Plant Sci Innovat, Lincoln, NE 68588 USA 3.Univ Nebraska, Sch Biol Sci, Lincoln, NE 68588 USA 4.Univ Nebraska, Dept Plant Pathol, Lincoln, NE 68583 USA 5.Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Li, Shengjun,Liu, Kan,Zhou, Bangjun,et al. MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis[J]. PLANT CELL,2018,30(2):481-494.
APA	Li, Shengjun.,Liu, Kan.,Zhou, Bangjun.,Li, Mu.,Zhang, Shuxin.,...&Yu, Bin.(2018).MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis.PLANT CELL,30(2),481-494.
MLA	Li, Shengjun,et al."MAC3A and MAC3B, Two Core Subunits of the MOS4-Associated Complex, Positively Influence miRNA Biogenesis".PLANT CELL 30.2(2018):481-494.