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Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source
Xu, Xin1,2; Xie, Meng1; Zhao, Qian3; Xian, Mo1; Liu, Huizhou1
2018
发表期刊BIOENGINEERED
ISSN2165-5979
卷号9期号:1页码:116-123
摘要We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06g L-1 mevalonate with a productivity of 0.03g L-1 h(-1) in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7.85g L-1 and 0.13g L-1 h(-1), respectively. Fed-batch fermentation was further optimized under anaerobic and microaerobic conditions, and mevalonate concentrations reached 3.05g L-1 and 4.97g L-1, respectively, indicating that the oxygen supply exerts a large impact on mevalonate production from acetate. This study describes a method with high potential to produce mevalonate with the engineered E. coli strain XU143 using the less costly alternative feedstock acetate as a carbon source.
文章类型Article
关键词Acetate Assimilation Aeration Mevalonate Two-stage Fed-batch Escherichia Coli
WOS标题词Science & Technology ; Life Sciences & Biomedicine
DOI10.1080/21655979.2017.1323592
关键词[WOS]ACETATE ACCUMULATION ; GROWTH ; EXPRESSION ; PROTEIN ; FLUX
收录类别SCI
语种英语
WOS研究方向Biotechnology & Applied Microbiology
项目资助者National Natural Science Foundation of China(21572242 ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; 21376255)
WOS类目Biotechnology & Applied Microbiology
WOS记录号WOS:000425053900002
出版者TAYLOR & FRANCIS INC
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文献类型期刊论文
条目标识符http://ir.qibebt.ac.cn/handle/337004/10634
专题生物基材料组群
通讯作者Xian, Mo; Liu, Huizhou
作者单位1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao, Peoples R China
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Xu, Xin,Xie, Meng,Zhao, Qian,et al. Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source[J]. BIOENGINEERED,2018,9(1):116-123.
APA Xu, Xin,Xie, Meng,Zhao, Qian,Xian, Mo,&Liu, Huizhou.(2018).Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source.BIOENGINEERED,9(1),116-123.
MLA Xu, Xin,et al."Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source".BIOENGINEERED 9.1(2018):116-123.
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