Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source | |
Xu, Xin1,2; Xie, Meng1; Zhao, Qian3; Xian, Mo1; Liu, Huizhou1 | |
2018 | |
发表期刊 | BIOENGINEERED |
ISSN | 2165-5979 |
卷号 | 9期号:1页码:116-123 |
摘要 | We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1.06g L-1 mevalonate with a productivity of 0.03g L-1 h(-1) in a 5-L bioreactor. The mevalonate concentration and productivity were significantly enhanced with increased cell density during 2-stage aerobic fermentation, reaching 7.85g L-1 and 0.13g L-1 h(-1), respectively. Fed-batch fermentation was further optimized under anaerobic and microaerobic conditions, and mevalonate concentrations reached 3.05g L-1 and 4.97g L-1, respectively, indicating that the oxygen supply exerts a large impact on mevalonate production from acetate. This study describes a method with high potential to produce mevalonate with the engineered E. coli strain XU143 using the less costly alternative feedstock acetate as a carbon source. |
文章类型 | Article |
关键词 | Acetate Assimilation Aeration Mevalonate Two-stage Fed-batch Escherichia Coli |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
DOI | 10.1080/21655979.2017.1323592 |
关键词[WOS] | ACETATE ACCUMULATION ; GROWTH ; EXPRESSION ; PROTEIN ; FLUX |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biotechnology & Applied Microbiology |
项目资助者 | National Natural Science Foundation of China(21572242 ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; 21376255) |
WOS类目 | Biotechnology & Applied Microbiology |
WOS记录号 | WOS:000425053900002 |
出版者 | TAYLOR & FRANCIS INC |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.qibebt.ac.cn/handle/337004/10634 |
专题 | 生物基材料组群 |
通讯作者 | Xian, Mo; Liu, Huizhou |
作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao, Peoples R China |
推荐引用方式 GB/T 7714 | Xu, Xin,Xie, Meng,Zhao, Qian,et al. Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source[J]. BIOENGINEERED,2018,9(1):116-123. |
APA | Xu, Xin,Xie, Meng,Zhao, Qian,Xian, Mo,&Liu, Huizhou.(2018).Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source.BIOENGINEERED,9(1),116-123. |
MLA | Xu, Xin,et al."Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source".BIOENGINEERED 9.1(2018):116-123. |
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